Low-level Laser (Light) Therapy Increases Mitochondrial Membrane Potential and ATP Synthesis in C2C12 Myotubes with a Peak Response at 3-6 h

Autor(es)

Ferraresi, Cleber ^{[1, 2, 3, 4]} ; Kaippert, Beatriz ^{[1, 5]} ; Avci, Pinar ^{[1, 6]} ; Huang, Ying-Ying ^{[1, 6]} ; de Sousa, Marcelo V. P. ^{[1, 7]} ; Bagnato, Vanderlei S. ^{[3, 4]} ; Parizotto, Nivaldo A. ^{[2, 3]} ; Hamblin, Michael R. ^{[1, 6, 8]}

Afiliação do(s) autor(es)

^[1] Massachusetts Gen Hosp, Wellman Ctr Photomed, Boston, MA 02114 - USA

^[2] Univ Fed Sao Carlos, Dept Phys Therapy, Lab Electrothermophototherapy, BR-13560 Sao Carlos, SP - Brazil

^[3] Univ Fed Sao Carlos, Postgrad Program Biotechnol, BR-13560 Sao Carlos, SP - Brazil

^[4] Univ Sao Paulo, Phys Inst Sao Carlos, Opt Grp, Sao Carlos, SP - Brazil

^[5] Univ Fed Rio de Janeiro, Rio De Janeiro, RJ - Brazil

^[6] Harvard Univ, Sch Med, Dept Dermatol, Boston, MA 02115 - USA

^[7] Univ Sao Paulo, Inst Phys, Lab Radiat Dosimetry & Med Phys, Sao Carlos, SP - Brazil

^[8] Harvard MIT Div Hlth Sci & Technol, Cambridge, MA - USA

• Tipo de documento: Artigo Científico
• Fonte: Photochemistry and Photobiology; v. 91, n. 2, p. 411-416, MAR-APR 2015.
• Citações Web of Science: 35

Resumo

Low-level laser (light) therapy has been used before exercise to increase muscle performance in both experimental animals and in humans. However, uncertainty exists concerning the optimum time to apply the light before exercise. The mechanism of action is thought to be stimulation of mitochondrial respiration in muscles, and to increase adenosine triphosphate (ATP) needed to perform exercise. The goal of this study was to investigate the time course of the increases in mitochondrial membrane potential (MMP) and ATP in myotubes formed from C2C12 mouse muscle cells and exposed to light-emitting diode therapy (LEDT). LEDT employed a cluster of LEDs with 20 red (630 +/- 10nm, 25mW) and 20 near-infrared (850 +/- 10nm, 50mW) delivering 28mWcm(2) for 90s (2.5Jcm(2)) with analysis at 5min, 3h, 6h and 24h post-LEDT. LEDT-6h had the highest MMP, followed by LEDT-3h, LEDT-24h, LEDT-5min and Control with significant differences. The same order (6h>3h>24h>5min> Control) was found for ATP with significant differences. A good correlation was found (r=0.89) between MMP and ATP. These data suggest an optimum time window of 3-6h for LEDT stimulate muscle cells. (AU)

• Processo FAPESP: 12/05919-0 - Efeitos do laser de baixa intensidade sobre a modulação do transcriptoma, imuno-histoquímica do tecido muscular e o desempenho físico de homens jovens submetidos a treinamento físico de força
• Beneficiário: Cleber Ferraresi
• Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 10/07194-7 - Uso das terapias laser e LED (Light-Emitting Diode) de baixa intensidade para aumentar o desempenho muscular: do in vitro e experimental ao uso clínico
• Beneficiário: Cleber Ferraresi
• Linha de fomento: Bolsas no Brasil - Doutorado