Identification and characterization of regions of difference between the Salmonella Gallinarum biovar Gallinarum and the Salmonella Gallinarum biovar Pullorum genomes

Autor(es)

Alves Batista, Diego Felipe ^[1] ; Freitas Neto, Oliveiro Caetano ^{[1, 2]} ; Barrow, Paul Andrew ^[3] ; de Oliveira, Marcos Tulio ^[4] ; Almeida, Adriana Maria ^[1] ; Ferraudo, Antonio Sergio ^[5] ; Berchieri, Jr., Angelo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Fac Ciencias Agr & Vet, Dept Patol Vet, BR-14884900 Sao Paulo - Brazil

^[2] Univ Fed Paraiba, Ctr Ciencias Agr, Dept Ciencias Vet, BR-58071680 Areia, Paraiba - Brazil

^[3] Univ Nottingham, Sch Vet Med & Sci, Loughborough LE12 5RD, Leics - England

^[4] Univ Estadual Paulista, Fac Ciencias Agr & Vet, Dept Tecnol, BR-14884900 Sao Paulo - Brazil

^[5] Univ Estadual Paulista, Fac Ciencias Agr & Vet, Dept Ciencias Exatas, BR-14884900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INFECTION GENETICS AND EVOLUTION; v. 30, p. 74-81, MAR 2015.
• Citações Web of Science: 8

Resumo

Salmonella Gallinarum is the causative agent of fowl typhoid, a severe septicaemic disease that affects birds of all ages, whereas S. Pullorum causes pullorum disease, a systemic disorder affecting primarily young birds. A proportion of birds with pullorum disease become carriers and are thereby able to transmit S. Pullorum vertically. Although these two pathogens cause distinct diseases, they are otherwise phenotypically and genetically similar. Therefore, the small variations that lead to the differences in virulence must have a genetic basis which currently is unknown. In the present study, we compared the genome sequences of S. Gallinarum (strains: SG287/91 and SG9) and S. Pullorum (strains: SP\_CDC, SP\_RKS, SP\_FCAV, SP\_S06) and identified 223 regions of difference (RODS), characterized by indels which were detected by using the software Artemis Comparison Tool. Some of the RODS led to pseudogenes frequently formed by frameshifts and premature stop codons in genes primarily involved in virulence and metabolism. We further verified the presence of some conserved RODs by PCR in 26 isolates of S. Gallinarum and 17 of S. Pullorum in order to extrapolate data analyses from genome comparison to field strains. The variations observed in virulence-related genes of S. Gallinarum and S. Pullorum appear not to be sufficient to explain the differences between the distinct biology of infection of fowl typhoid and pullorum disease. Thus, we suggest that the identified pseudogenes affecting metabolism might play a greater role during infection than previously thought. (C) 2014 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 11/23483-1 - Salmonella Gallinarum (SG): 1. Comparação do genoma de Sg e S. Pullorum. 2. Sequenciamento de mutante atenuado (Sg "cobS" cbiA) e análise do transcriptoma em macrófagos de aves. 3. Patogenia de estirpes com e sem flagelos
• Beneficiário: Angelo Berchieri Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/02253-6 - Investigando as alterações metabólicas causadas pela expressão transgênica da oxidase alternativa mitocondrial de Ciona intestinalis em Drosophila melanogaster
• Beneficiário: Marcos Túlio de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores