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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Digestive peptidase evolution in holometabolous insects led to a divergent group of enzymes in Lepidoptera

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Autor(es):
Dias, Renata O. [1] ; Via, Allegra [2, 3] ; Brandao, Marcelo M. [4] ; Tramontano, Anna [2, 3] ; Silva-Filho, Marcio C. [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Genet, BR-13418900 Piracicaba, SP - Brazil
[2] Univ Roma La Sapienza, Fdn Cenci Bolognetti, Dept Phys, I-00185 Rome - Italy
[3] Univ Roma La Sapienza, Fdn Cenci Bolognetti, Ist Pasteur, I-00185 Rome - Italy
[4] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, BR-13083875 Campinas, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Insect Biochemistry and Molecular Biology; v. 58, p. 1-11, MAR 2015.
Citações Web of Science: 5
Resumo

Trypsins and chymotrypsins are well-studied serine peptidases that cleave peptide bonds at the carboxyl side of basic and hydrophobic L-amino acids, respectively. These enzymes are largely responsible for the digestion of proteins. Three primary processes regulate the activity of these peptidases: secretion, precursor (zymogen) activation and substrate-binding site recognition. Here, we present a detailed phylogenetic analysis of trypsins and chymotrypsins in three orders of holometabolous insects and reveal divergent characteristics of Lepidoptera enzymes in comparison with those of Coleoptera and Diptera. In particular, trypsin subsite S1 was more hydrophilic in Lepidoptera than in Coleoptera and Diptera, whereas subsites S2-S4 were more hydrophobic, suggesting different substrate preferences. Furthermore, Lepidoptera displayed a lineage-specific trypsin group belonging only to the Noctuidae family. Evidence for facilitated trypsin auto-activation events were also observed in all the insect orders studied, with the characteristic zymogen activation motif complementary to the trypsin active site. In contrast, insect chymotrypsins did not seem to have a peculiar evolutionary history with respect to their mammal counterparts. Overall, our findings suggest that the need for fast digestion allowed holometabolous insects to evolve divergent groups of peptidases with high auto-activation rates, and highlight that the evolution of trypsins led to a most diverse group of enzymes in Lepidoptera. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 10/17110-5 - Caracterização das bases moleculares envolvidas no polifagismo de insetos herbívoros
Beneficiário:Renata de Oliveira Dias
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 11/00417-3 - Biologia de sistemas aplicada a agricultura: análise de transcriptomas e interactomas
Beneficiário:Marcelo Mendes Brandao
Linha de fomento: Auxílio à Pesquisa - Jovens Pesquisadores
Processo FAPESP: 08/52067-3 - Herbivoria e o transporte intracelular de proteínas
Beneficiário:Márcio de Castro Silva Filho
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Temático
Processo FAPESP: 12/03040-0 - Evolução e caracterização da família gênica das serina proteinases e sua associação ao comportamento alimentar em insetos da família Noctuidae
Beneficiário:Renata de Oliveira Dias
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Doutorado