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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Pentoxifylline enhances the protective effects of hypertonic saline solution on liver ischemia reperfusion injury through inhibition of oxidative stress

Texto completo
Autor(es):
Rocha-Santos, Vinicius [1] ; Figueira, Estela R. R. [1] ; Rocha-Filho, Joel A. [2] ; Coelho, Ana M. M. [1] ; Pinheiro, Rafael Soraes [1] ; Bacchella, Telesforo [1] ; Machado, Marcel C. C. [1] ; D'Albuquerque, Luiz A. C. [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Med, Hosp Clin, Dept Gastroenterol, Lab Med Invest LIM37, Disciplin, BR-04001083 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Sch Med, Discipline Anesthesiol, Hosp Clin, BR-04001083 Sao Paulo, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Hepatobiliary & Pancreatic Diseases International; v. 14, n. 2, p. 194-200, APR 15 2014.
Citações Web of Science: 6
Resumo

BACKGROUND: Liver ischemia reperfiision (IR) injury triggers a systemic inflammatory response and is the main cause of organ dysfunction and adverse postoperative outcomes after liver surgery. Pentoxifylline (PTX) and h-ypertonic saline solution (HTS) have been identified to have beneficial effects against IR injury This study aimed to investigate if the addition of PTX to HTS is superior to HTS alone for the prevention of liver IR injury. METHODS: Male Wistar rats were allocated into three groups. Control rats underwent 60 minutes. of partial liver ischemia, HTS rats were treated with 0.4 mL/kg of intravenous 7.5% NaC1 15 minutes before reperfusion, and HPTX group were treated with 7.5% NaC1 plus 25 mg/kg of PTX 15 minutes before reperfusion. Samples were collected after reperfusion for determination of ALT, AST, TNF-alpha, IL-6, IL-10, mitochondrial respiration, lipid peroxidation, pulmonary permeability and myeloperoxidase. RESULTS: HPTX significantly decreased TNF-alpha 30 minutes after reperfusion. HPTX and HTS significantly decreased ALT, AST, IL-6, mitochondrial dysfunction and pulmonary myeloperoxidase 4 hours after reperfusion. Compared with HTS only, HPTX significantly decreased hepatic oxidative stress 4 hours after reperfusion and pulmonary permeability 4 and 12 hours after reperfusion. CONCLUSION: This study showed that PTX added the beneficial effects of HTS on liver IR injury through decreases of hepatic oxidative stress and pulmonary permeability. (AU)

Processo FAPESP: 11/05214-3 - Efeitos do pré-condicionamento isquêmico na hemodinâmica e no metabolismo hepático em modelo experimental de isquemia/reperfusão do fígado em ratos
Beneficiário:Estela Regina Ramos Figueira
Linha de fomento: Auxílio à Pesquisa - Regular