Pentoxifylline enhances the protective effects of hypertonic saline solution on liver ischemia reperfusion injury through inhibition of oxidative stress

Autor(es)

Rocha-Santos, Vinicius ^[1] ; Figueira, Estela R. R. ^[1] ; Rocha-Filho, Joel A. ^[2] ; Coelho, Ana M. M. ^[1] ; Pinheiro, Rafael Soraes ^[1] ; Bacchella, Telesforo ^[1] ; Machado, Marcel C. C. ^[1] ; D'Albuquerque, Luiz A. C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med, Hosp Clin, Dept Gastroenterol, Lab Med Invest LIM37, Disciplin, BR-04001083 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Sch Med, Discipline Anesthesiol, Hosp Clin, BR-04001083 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Hepatobiliary & Pancreatic Diseases International; v. 14, n. 2, p. 194-200, APR 15 2014.
• Citações Web of Science: 6

Resumo

BACKGROUND: Liver ischemia reperfiision (IR) injury triggers a systemic inflammatory response and is the main cause of organ dysfunction and adverse postoperative outcomes after liver surgery. Pentoxifylline (PTX) and h-ypertonic saline solution (HTS) have been identified to have beneficial effects against IR injury This study aimed to investigate if the addition of PTX to HTS is superior to HTS alone for the prevention of liver IR injury. METHODS: Male Wistar rats were allocated into three groups. Control rats underwent 60 minutes. of partial liver ischemia, HTS rats were treated with 0.4 mL/kg of intravenous 7.5% NaC1 15 minutes before reperfusion, and HPTX group were treated with 7.5% NaC1 plus 25 mg/kg of PTX 15 minutes before reperfusion. Samples were collected after reperfusion for determination of ALT, AST, TNF-alpha, IL-6, IL-10, mitochondrial respiration, lipid peroxidation, pulmonary permeability and myeloperoxidase. RESULTS: HPTX significantly decreased TNF-alpha 30 minutes after reperfusion. HPTX and HTS significantly decreased ALT, AST, IL-6, mitochondrial dysfunction and pulmonary myeloperoxidase 4 hours after reperfusion. Compared with HTS only, HPTX significantly decreased hepatic oxidative stress 4 hours after reperfusion and pulmonary permeability 4 and 12 hours after reperfusion. CONCLUSION: This study showed that PTX added the beneficial effects of HTS on liver IR injury through decreases of hepatic oxidative stress and pulmonary permeability. (AU)

• Processo FAPESP: 11/05214-3 - Efeitos do pré-condicionamento isquêmico na hemodinâmica e no metabolismo hepático em modelo experimental de isquemia/reperfusão do fígado em ratos
• Beneficiário: Estela Regina Ramos Figueira
• Modalidade de apoio: Auxílio à Pesquisa - Regular