Adipose tissue-derived stem cell therapy in rat cryopreserved ovarian grafts

Autor(es)

Damous, Luciana Lamarao ^[1] ; Nakamuta, Juliana Sanajotti ^[2] ; Teofilo Saturi de Carvalho, Ana Elisa ^[2] ; Soares-, Jr., Jose Maria ^[1] ; Simoes, Manuel de Jesus ^[3] ; Krieger, Jose Eduardo ^[2] ; Baracat, Edmund C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med, Hosp Clin, Lab Struct & Mol Gynecol LIM 58, Gynecol Disciplin, BR-01246903 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Med, Heart Inst Incor, Lab Genet & Mol Cardiol, BR-05403000 Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo UNIFESP, Dept Morphol & Genet, BR-04023009 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: STEM CELL RESEARCH & THERAPY; v. 6, APR 15 2015.
• Citações Web of Science: 12

Resumo

The preliminary results of ovarian transplantation in clinical practice are encouraging. However, the follicular depletion caused by ischemic injury is a main concern and is directly related to short-term graft survival. Cell therapy with adipose tissue-derived stem cells (ASCs) could be an alternative to induce early angiogenesis in the graft. This study aimed to evaluate ASCs therapy in rat cryopreserved ovarian grafts. A single dose of rat ASC (rASCs) or vehicle was injected into the bilateral cryopreserved ovaries of twelve adult female rats immediately after an autologous transplant. Daily vaginal smears were performed for estrous cycle evaluation until euthanasia on postoperative day 30. Follicle viability, graft morphology and apoptosis were assessed. No differences were found with respect to estrous cycle resumption and follicle viability (P > 0.05). However, compared with the vehicle-treated grafts, the morphology of the ASCs-treated grafts was impaired, with diffuse atrophy and increased apoptosis (P < 0.05). ASCs direct injected in the stroma of rat cryopreserved ovarian grafts impaired its morphology although may not interfere with the functional resumption on short-term. Further investigations are necessary to evaluated whether it could compromise their viability in the long-term. (AU)

• Processo FAPESP: 12/09469-9 - Terapia celular e gênica no transplante experimental de ovário fresco e criopreservado
• Beneficiário: Edmund Chada Baracat
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/17897-5 - Terapia celular e gênica no transplante experimental de ovário fresco e criopreservado
• Beneficiário: Luciana Lamarão Damous
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado