Analysis of Metabolic Changes in Plant Pathosystems by Imprint Imaging DESI-MS

Autor(es)

Tata, Alessandra ^{[1, 2]} ; Perez, Consuelo J. ^[1] ; Hamid, Tanam S. ^[1] ; Bayfield, Mark A. ^[3] ; Ifa, Demian R. ^[1]

Afiliação do(s) autor(es)

^[1] York Univ, Dept Chem, Ctr Res Mass Spectrometry, Toronto, ON M3J 2R7 - Canada

^[2] Univ Campinas UNICAMP, Inst Chem, ThoMSon Mass Spectrometry Lab, Sao Paulo - Brazil

^[3] York Univ, Dept Biol, Toronto, ON M3J 2R7 - Canada

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY; v. 26, n. 4, p. 641-648, APR 2015.
• Citações Web of Science: 19

Resumo

The response of plants to microbial pathogens is based on the production of secondary metabolites. The complexity of plant-pathogen interactions makes their understanding a challenging task for metabolomic studies requiring powerful analytical approaches. In this paper, the ability of ambient mass spectrometry to provide a snapshot of plant metabolic response to pathogen invasion was tested. The fluctuations of glycoalkaloids present in sprouted potatoes infected by the phytopathogen Pythium ultimum were monitored by imprint imaging desorption electrospray ionization mass spectrometry (DESI-MS). After 8 d from the inoculation, a decrease of the relative abundance of potato glycoalkaloids alpha-solanine (m/z 706) and alpha-chaconine (m/z 722) was observed, whereas the relative intensity of solanidine (m/z 398), solasodenone (m/z 412), solanaviol (m/z 430), solasodiene (m/z 396), solaspiralidine (m/z 428), gamma-solanine/gamma-chaconine (m/z 560) , beta-solanine (m/z 706), and beta-chaconine (m/z 722) increased. The progression of the disease, expressed by the development of brown necrotic lesions on the potato, led to the further decrease of all the glycoalkaloid metabolites. Therefore, the applicability of imprint imaging DESI-MS in studying the plant metabolic changes in a simple pathosystem was demonstrated with minimal sample preparation. (AU)

• Processo FAPESP: 13/15575-9 - Procura e mapeamento de quorum sensing em fungos por espectrometria de massas em condições ambientes: determinação do perfil químico e localização de moléculas envolvidas em biocontrole entre agentes fúngicos e diferentes patógenos
• Beneficiário: Alessandra Tata
• Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado