| Texto completo | |
| Autor(es): |
Cecchetto, Juliana
[1]
;
Carvalho, Fernanda C.
[1]
;
Santos, Adriano
[1]
;
Fernandes, Flavio C. B.
[1]
;
Bueno, Paulo R.
[2]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Sao Paulo State Univ UNESP, Dept Phys Chem, Inst Chem, BR-14800060 Sao Paulo - Brazil
[2] Sao Paulo State Univ UNESP, Dept Phys Chem, Inst Chem, Phys, UNESP, BR-14800060 Sao Paulo - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | SENSORS AND ACTUATORS B-CHEMICAL; v. 213, p. 150-154, JUL 5 2015. |
| Citações Web of Science: | 31 |
| Resumo | |
NS1, a non-structural dengue protein, has been used as a biomarker for the detection of viremia in dengue diagnosis. In this work, an impedimetric label-free immunosensor based on an anti-NS1 modified gold electrode was developed. To immobilize anti-NS1, a mixed self-assembled monolayer consisting of 11-mercaptoundecanoic acid (for covalent anti-NS1 attachment) and 6-mercaptohexanol (as a spacer) was prepared. The impedance spectra were recorded in the presence of a redox probe ( {[}Fe(CN)(6)](3-/4-)) to monitor changes in charge-transfer resistance associated with target binding both in PBS and neat serum. A calibration curve was constructed in order to obtain analytical parameters. NS1 was diluted in PBS to final concentrations in the linear range of 0.01-2.00 mu g mL(-1) with a sensitivity of 14.1 percentage decade(-1) (R similar to 0.99) and a limit of detection (LOD) of 3 ng mL(-1). A linear range 0.01-1.00 mu g mL(-1) of NS1 diluted in neat serum was also obtained (R similar to 0.98) with a sensitivity of 10.4 percentage decade(-1) and a LOD of 30 ng mL(-1). The results open new insights into the potential use of this biosensor for point-of-care and bedside applications for dengue diagnosis. (C) 2015 Elsevier B.V. All rights reserved. (AU) | |