Behavior of Listeria monocytogenes in a multi-species biofilm with Enterococcus faecalis and Enterococcus faecium and control through sanitation procedures

Autor(es)

Fernandes, Meg da Silva ^[1] ; Kabuki, Dirce Yorika ^[2] ; Kuaye, Arnaldo Yoshiteru ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Dept Food Technol, Campinas, SP - Brazil

^[2] Univ Campinas UNICAMP, Dept Food Sci, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Food Microbiology; v. 200, p. 5-12, MAY 4 2015.
• Citações Web of Science: 11

Resumo

The formation of mono-species biofilm (Listeria monocytogenes) and multi-species biofilms (Enterococcus faecium, Enterococcus faecalis, and L. monocytogenes) was evaluated. In addition, the effectiveness of sanitation procedures for the control of the multi-species biofilm also was evaluated. The biofilms were grown on stainless steel coupons at various incubation temperatures (7, 25 and 39 degrees C) and contact times (0, 1, 2, 4, 6 and 8 days). In all tests, at 7 degrees C, the microbial counts were below 0.4 log CFU/cm(2) and not characteristic of biofilms. In mono-species biofilm, the counts of L. monocytogenes after 8 days of contact were 4.1 and 2.8 log CFU/cm(2) at 25 and 39 degrees C, respectively. In the multi-species biofilms, Enterococcus spp. were present at counts of 8 log CFU/cm(2) at 25 and 39 degrees C after 8 days of contact. However, the L. monocytogenes in multi-species biofilms was significantly affected by the presence of Enterococcus spp. and by temperature. At 25 degrees C, the growth of L. monocytogenes biofilms was favored in multi-species cultures, with counts above 6 log CFU/cm(2) after 8 days of contact. In contrast, at 39 degrees C, a negative effect was observed for L. monocytogenes biofilm growth in mixed cultures, with a significant reduction in counts over time and values below 0.4 log CFU/cm(2) starting at day 4. Anionic tensioactive cleaning complemented with another procedure (acid cleaning, disinfection or acid cleaning + disinfection) eliminated the multi-species biofilms under all conditions tested (counts of all micro-organisms < 0.4 log CFU/cm(2)). Peracetic acid was the most effective disinfectant, eliminating the multi-species biofilms under all tested conditions (counts of the all microorganisms <0.4 log CFU/cm(2)). In contrast, biguanide was the least effective disinfectant, failing to eliminate biofilms under all the test conditions. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 10/10507-7 - FORMAÇÃO DE BIOFILMES, PATOGENICIDADE E QUORUM-SENSING EM CEPAS DE E. faecium E E. faecalis ISOLADAS NO PROCESSAMENTO DE RICOTA.
• Beneficiário: Meg da Silva Fernandes
• Modalidade de apoio: Bolsas no Brasil - Doutorado