Down-regulation of the CYP19A1 gene in cumulus cells of infertile women with endometriosis

Autor(es)

Barcelos, Ionara Diniz E. S. ^[1] ; Donabella, Flavia Capello ^[1] ; Ribas, Cristiana Padovan ^[1] ; Meola, Juliana ^[1] ; Ferriani, Rui Alberto ^[1] ; Paro de Paz, Claudia Cristina ^[2] ; Navarro, Paula A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Gynecol & Obstet, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Genet, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Reproductive BioMedicine Online; v. 30, n. 5, p. 532-541, MAY 2015.
• Citações Web of Science: 12

Resumo

Aromatase plays a fundamental role in the establishment of oocyte quality, which might be compromised in infertile women with endometriosis. The expression of the CYP19A1 gene (that encodes aromatase) was compared in cumulus cells and oestradiol concentrations in the follicular fluid of infertile women with and without endometriosis submitted to ovarian stimulation for intracytoplasmic sperm injection. Cumulus cells were isolated and the expression of the CYP19A1 was quantitated through real-time polymerase chain reaction. Oestradiol concentrations in follicular fluid were measured by chemiluminescence immunoassay. A lower expression of the CYP19A1 in the cumulus cells of infertile women with endometriosis was observed compared with controls (0.17 +/- 0.13 and 0.56 +/- 0.12, respectively), and no significant difference in the follicular fluid oestradiol concentrations was observed between groups. Our results show reduced expression of the CYP19A1 in cumulus cells of infertile women with endometriosis, which may play a role in the pathogenesis of endometriosis-related infertility. (C) 2015 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 08/58197-6 - Balanço oxidante e antioxidante de mulheres inférteis com endometriose comparadas a controle (fatores masculino e tubário de infertilidade) submetidas a estimulação ovariana e influência nos resultados
• Beneficiário: Paula Andrea de Albuquerque Salles Navarro
• Modalidade de apoio: Auxílio à Pesquisa - Regular