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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Bacillus subtilis spores as adjuvants for DNA vaccines

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Autor(es):
Aps, Luana R. M. M. [1] ; Diniz, Mariana O. [1] ; Porchia, Bruna F. M. M. [1] ; Sales, Natiely S. [1] ; Moreno, Ana Carolina R. [1] ; Ferreira, Luis C. S. [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Vaccine Dev Lab, Sao Paulo - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: Vaccine; v. 33, n. 20, p. 2328-2334, MAY 11 2015.
Citações Web of Science: 7
Resumo

Recently, Bacillus subtilis spores were shown to be endowed with strong adjuvant capacity when co-administered with purified antigenic proteins. In the present study we assessed whether spores possess adjuvant properties when combined with DNA vaccines. We showed that B. subtilis spores promoted the activation of dendritic cells in vitro and induced migration of pro-inflammatory cells after parenteral administration to mice. Likewise, co-administration of spores with a DNA vaccine encoding the human papillomavirus type 16 (HPV-16) E7 protein enhanced the activation of antigen-specific CD8(+) T cell responses in vivo. Mice immunized with the DNA vaccine admixed with spores presented a protective immunity increase to previously implanted tumor cells, capable of expressing HPV-16 oncoproteins. Finally, we observed that the adjuvant effect can vary accordingly to the number of co-administered spores which may be ascribed with the ability to induce. Collectively, the present results demonstrate for the first time that B. subtilis spores can also confer adjuvant effects to DNA vaccines. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 11/13805-1 - Efeito adjuvante de esporos de Bacillus subtilis coadministrados a vacinas de DNA
Beneficiário:Luana Raposo de Melo Moraes Aps
Linha de fomento: Bolsas no Brasil - Mestrado