Determination of carbon in digested samples and amino acids by inductively coupled plasma tandem mass spectrometry

Autor(es)

Amaral, Clarice D. B. ^{[1, 2]} ; Amais, Renata S. ^[3] ; Fialho, Lucimar L. ^[1] ; Schiavo, Daniela ^[4] ; Nogueira, Ana Rita A. ^[2] ; Nobrega, Joaquim A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Chem, Grp Appl Instrumental Anal, BR-13565905 Sao Carlos, SP - Brazil

^[2] Embrapa Southeast Livestock, BR-13560970 Sao Carlos, SP - Brazil

^[3] Univ Sao Paulo, Ctr Nucl Energy Agr, BR-13416000 Piracicaba, SP - Brazil

^[4] Agilent Technol Brazil, BR-06460040 Barueri, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Microchemical Journal; v. 122, p. 29-32, SEP 2015.
• Citações Web of Science: 14

Resumo

Inductively coupled plasma mass spectrometer with a triple quadrupole (ICP-MS/MS) was employed to determine carbon from different sources such as plant digests, amino acids and peptides. Adequate precision, accuracy and sensitivity were obtained when using either single quadrupole or mass shift mode. Recoveries for carbon determination in plant digests ranged from 96 to 100%. Limits of detection were 0.42 and 0.17 mg L-1 for C-12(+) and (CO+,)-C-12-O-16 respectively, with calibration curves generated by using solutions prepared with oxalic acid. The determination of carbon and sulfur in amino acids and peptides showed that accuracy was better when measuring in O-2 reaction mode. Additionally, C and S were simultaneously determined in cystine and glutathione by ICP-MS/MS mass-shift mode. The determination of carbon simultaneously with trace elements and target elements by ICP-MS makes this concept quite promising. Results demonstrated the potential of ICP-MS/MS for carbon determination. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 13/26857-5 - Teores totais e análise de especiação química de As, Cr e Se usando colunas monolíticas a baixa pressão e técnicas com plasma acoplado indutivamente
• Beneficiário: Renata Stábile Amais
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado