Biomodulation of Inflammatory Cytokines Related to Oral Mucositis by Low-Level Laser Therapy

Autor(es)

Basso, Fernanda G. ^[1] ; Pansani, Taisa N. ^[1] ; Soares, Diana G. ^[1] ; Scheffel, Debora L. ^[1] ; Bagnato, Vanderlei S. ^[2] ; de Souza Costa, Carlos Alberto ^[1] ; Hebling, Josimeri ^[1]

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Araraquara Sch Dent, Araraquara - Brazil

^[2] Univ Sao Paulo, Sao Carlos Phys Inst, Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Photochemistry and Photobiology; v. 91, n. 4, p. 952-956, JUL-AUG 2015.
• Citações Web of Science: 17

Resumo

This study evaluated the effects of LLLT on the expression of inflammatory cytokines related to the development of oral mucositis by gingival fibroblasts. Primary gingival fibroblasts were seeded on 24-well plates (10(5) cells/well) for 24h. Fresh serum-free culture medium (DMEM) was then added, and cells were placed in contact with LPS (Escherichia coli, 1gmL(-1)), followed by LLLT irradiation (LaserTABLEInGaAsP diode prototype780nm, 25mW) delivering 0, 0.5, 1.5 or 3Jcm(-2). Cells without contact with LPS were also irradiated with the same energy densities. Gene expression of TNF-, IL-1, IL-6 and IL-8 was evaluated by Real-Time PCR, and protein synthesis of these cytokines was determined by enzyme-linked immunosorbent (ELISA) assay. Data were statistically analyzed by the Kruskal-Wallis test, complemented by the Mann-Whitney test (P<0.05). LPS treatment increased the gene expression and protein synthesis of TNF-, IL-6 and IL-8, while the expression of IL-1 was not affected. For LPS-treated groups, LLLT promoted significant decreases in the expression of TNF-, IL-6, and IL-8 at 1.5Jcm(-2) and 3Jcm(-2). These results demonstrate that LLLT promoted a beneficial biomodulatory effect on the expression of inflammatory cytokines related to oral mucositis by human gingival fibroblasts. (AU)

• Processo FAPESP: 13/05879-0 - Efeito da laserterapia de baixa potência e fatores de crescimento sobre a atividade de fibroblastos de gengiva obtidos de pacientes jovens e idosos
• Beneficiário: Carlos Alberto de Souza Costa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/17947-8 - Efeito do laser de baixa intensidade sobre a expressão de citocinas inflamatórias na mucosite oral
• Beneficiário: Fernanda Gonçalves Basso
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/06057-7 - Desenvolvimento de modelo de cultura de células 3D de mucosa oral para estudos in vitro
• Beneficiário: Fernanda Gonçalves Basso
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado