Biomodulation of Inflammatory Cytokines Related to Oral Mucositis by Low-Level Laser Therapy

Author(s)

Basso, Fernanda G. ^[1] ; Pansani, Taisa N. ^[1] ; Soares, Diana G. ^[1] ; Scheffel, Debora L. ^[1] ; Bagnato, Vanderlei S. ^[2] ; de Souza Costa, Carlos Alberto ^[1] ; Hebling, Josimeri ^[1]

Affiliation

^[1] UNESP Univ Estadual Paulista, Araraquara Sch Dent, Araraquara - Brazil

^[2] Univ Sao Paulo, Sao Carlos Phys Inst, Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: Photochemistry and Photobiology; v. 91, n. 4, p. 952-956, JUL-AUG 2015.
• Web of Science Citations: 17

Abstract

This study evaluated the effects of LLLT on the expression of inflammatory cytokines related to the development of oral mucositis by gingival fibroblasts. Primary gingival fibroblasts were seeded on 24-well plates (10(5) cells/well) for 24h. Fresh serum-free culture medium (DMEM) was then added, and cells were placed in contact with LPS (Escherichia coli, 1gmL(-1)), followed by LLLT irradiation (LaserTABLEInGaAsP diode prototype780nm, 25mW) delivering 0, 0.5, 1.5 or 3Jcm(-2). Cells without contact with LPS were also irradiated with the same energy densities. Gene expression of TNF-, IL-1, IL-6 and IL-8 was evaluated by Real-Time PCR, and protein synthesis of these cytokines was determined by enzyme-linked immunosorbent (ELISA) assay. Data were statistically analyzed by the Kruskal-Wallis test, complemented by the Mann-Whitney test (P<0.05). LPS treatment increased the gene expression and protein synthesis of TNF-, IL-6 and IL-8, while the expression of IL-1 was not affected. For LPS-treated groups, LLLT promoted significant decreases in the expression of TNF-, IL-6, and IL-8 at 1.5Jcm(-2) and 3Jcm(-2). These results demonstrate that LLLT promoted a beneficial biomodulatory effect on the expression of inflammatory cytokines related to oral mucositis by human gingival fibroblasts. (AU)

• FAPESP's process: 13/05879-0 - Effect of low-level laser therapy and growth factors on gingival fibroblasts activity of young and aged patients
• Grantee: Carlos Alberto de Souza Costa
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/17947-8 - EFFECTS OF LOW-LEVEL LASER THERAPY ON INFLAMMATORY CYTOKINES EXPRESSION ON ORAL MUCOSITIS
• Grantee: Fernanda Gonçalves Basso
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 14/06057-7 - Development of a 3D oral mucosa model for in vitro studies
• Grantee: Fernanda Gonçalves Basso
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor