| Texto completo | |
| Autor(es): |
Cruz-Martinez, L. C.
[1]
;
Jesus, C. K. C.
[1]
;
Matsudo, M. C.
[2]
;
Danesi, E. D. G.
[3]
;
Sato, S.
[1]
;
Carvalho, J. C. M.
[1]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Biochem & Pharmaceut Technol, Sao Paulo, SP - Brazil
[2] Univ Fed Itajuba, Itajuba, MG - Brazil
[3] Univ Estadual Ponta Grossa, Dept Food Engn, Ponta Grossa, PR - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Brazilian Journal of Chemical Engineering; v. 32, n. 2, p. 347-356, APR-JUN 2015. |
| Citações Web of Science: | 4 |
| Resumo | |
AbstractNH4NO3 simultaneously provides a readily assimilable nitrogen source (ammonia) and a reserve of nitrogen (nitrate), allowing for an increase in Arthrospira platensis biomass production while reducing the cost of the cultivation medium. In this study, a 22plus star central composite experimental design combined with response surface methodology was employed to analyze the influence of light intensity (I) and the total amount of added NH4NO3 (Mt) on a bench-scale tubular photobioreactor for fed-batch cultures. The maximum cell concentration (Xm), cell productivity (PX) and biomass yield on nitrogen (YX/N) were evaluated, as were the protein and lipid contents. Under optimized conditions (I = 148 μmol·photons·m-2·s-1 and Mt = 9.7 mM NH4NO3), Xm = 4710 ±34.4 mg·L-1, PX = 478.9 ±3.8 mg·L-1·d-1 and YX/N = 15.87 ±0.13 mg·mg-1 were obtained. The best conditions for protein content in the biomass (63.2%) were not the same as those that maximized cell growth (I = 180 μmol·photons·m-2·s-1 and Mt = 22.5 mM NH4NO3). Based on these results, it is possible to conclude that ammonium nitrate is an interesting alternate nitrogen source for the cultivation of A. platensisin a fed-batch process and could be used for other photosynthetic microorganisms. (AU) | |
| Processo FAPESP: | 09/53265-6 - Cultivo descontínuo alimentado de Spirulina (Arthrospira) platensis em reator tubular utilizando nitrato de amônio como fonte de nitrogênio |
| Beneficiário: | João Carlos Monteiro de Carvalho |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |