Maternal Supplementation with Oligofructose (10%) during Pregnancy and Lactation Leads to Increased Pro-Inflammatory Status of the 21-D-Old Offspring

Autor(es)

Mennitti, Lais Vales ^[1] ; Oyama, Lila Missae ^[2] ; de Oliveira, Juliana Lopez ^[2] ; Losinskas Hachul, Ana Claudia ^[2] ; Santamarina, Aline Boveto ^[2] ; de Santana, Aline Alves ^[2] ; Okuda, Marcos Hiromu ^[2] ; Ribeiro, Eliane Beraldi ^[2] ; da Penha Oller do Nascimento, Claudia Maria ^[2] ; Pisani, Luciana Pellegrini ^[3]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Programa Pos Grad Interdisciplinar Ciencias Saude, Santos, SP - Brazil

^[2] Univ Fed Sao Paulo, Dept Fisiol, Disciplina Fisiol Nutr, Escola Paulista Med, Sao Paulo, SP - Brazil

^[3] Univ Fed Sao Paulo, Inst Saude Sociedade, Dept Biociencias, Santos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 10, n. 7 JUL 6 2015.
• Citações Web of Science: 3

Resumo

Previously, we showed that oligofructose (10%) supplementation during pregnancy and lactation increased endotoxemia in 21-d-old pups. The present study evaluated the effect of 10% oligofructose diet supplementation during pregnancy and lactation in the presence or absence of hydrogenated vegetable fat on the pro-inflammatory status of 21-d-old offspring. On the first day of pregnancy, female Wistar rats were divided into the following groups: control diet (C), control diet supplemented with 10% oligofructose (CF), diet enriched with hydrogenated vegetable fat (T) or diet enriched with hydrogenated vegetable fat supplemented with 10% oligofructose (TF). Diets were maintained during pregnancy and lactation. Serum TNF-alpha (tumor necrosis factor alpha) was assessed using a specific kit. Protein expression was determined by Western Blotting, and the relative mRNA levels were analyzed by RTPCR (real-time polymerase chain reaction). We observed that 10% oligofructose supplementation during pregnancy and lactation increased offspring's IL-6R (interleukin-6 receptor) mRNA levels in the liver and RET (retroperitoneal white adipose tissue) and decreased (adiponectin receptor 2) and ADIPOR1 (adiponectin receptor 1) gene expression in liver and EDL (extensor digital longus)/SOL (soleus) muscles of CF group. Additionally, TF group presented with increased serum TNF-alpha, protein expression of p-NF kappa Bp65 (phosphorylated form of nuclear factor kappa B p65 subunit) in liver and IL-6R mRNA levels in RET. These findings were accompanied by decreased levels of ADIPOR1 mRNA in the EDL and SOL muscles of the TF group. In conclusion, supplementing the dam's diet with 10% of oligofructose during pregnancy and lactation, independent of hydrogenated vegetable fat addition, contributes to the increased pro-inflammatory status of 21-d-old offspring, possibly through the activation of the TLR4 (toll like receptor 4) pathway. (AU)

• Processo FAPESP: 11/19426-2 - Efeito da ingestão de dieta enriquecida com prebióticos nos mecanismo intracelulares envolvidos com o desenvolvimento de resistência à insulina, na prole, com 21 e 90 dias de vida, de mães tratadas com ácidos graxos trans, durante a gestação e lactação
• Beneficiário: Luciana Pellegrini Pisani
• Modalidade de apoio: Auxílio à Pesquisa - Regular