Effect of a calcium hydroxide/chlorhexidine paste as intracanal dressing in human primary teeth with necrotic pulp against Porphyromonas gingivalis and Enterococcus faecalis

Autor(es)

Gondim, Juliana O. ^{[1, 2]} ; Avaca-Crusca, Juliana S. ^[3] ; Valentini, Sandro R. ^[3] ; Zanelli, Cleslei F. ^[3] ; Spolidorio, Denise M. P. ^[4] ; Giro, Elisa M. A. ^[5]

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Araraquara Dent Sch, Dept Orthodont & Pediat Dent, Araraquara, SP - Brazil

^[2] Univ Fed Ceara, Sch Dent, Sobral, CE - Brazil

^[3] UNESP Univ Estadual Paulista, Dept Biol Sci, Sch Pharmaceut Sci, Araraquara, SP - Brazil

^[4] UNESP Univ Estadual Paulista, Araraquara Dent Sch, Dept Physiol & Pathol, Araraquara, SP - Brazil

^[5] UNESP Univ Estadual Paulista, Dept Orthodont & Pediat Dent, Araraquara Dent Sch, Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Paediatric Dentistry; v. 22, n. 2, p. 116-124, MAR 2012.
• Citações Web of Science: 12

Resumo

Background. Intracanal medication is important for endodontic treatment success as it eliminates microorganisms that persist after biomechanical preparation. Aim. To evaluate the effect of two intracanal medications against Porphyromonas gingivalis and Enterococcus faecalis in the root canals of human primary teeth with necrotic pulp with and without furcal/periapical lesion, using quantitative real- time polymerase chain reaction (qRT-PCR). Design. Thirty-two teeth with necrotic pulp were used. Twelve teeth did not present lesion, and 20 teeth presented radiographically visible furca/periapical lesion. Microbiological samples were collected after coronal access and bio- mechanical preparation. The teeth were medicated with calcium hydroxide pastes prepared with either polyethylene glycol or chlorhexidine. After 30 days, the medication was removed and a third collection was performed. Microbiological samples were processed using qRT-PCR. Data were analysed by Wilcoxon and Mann-Whitney tests (alpha = 0.05). Results. There was no significant difference in the microbiota present in the primary teeth with and without furcal/periapical lesion. Biomechanical preparation was effective in reducing the number of microorganisms (P < 0.05). The intracanal medications had similar antibacterial activity. Conclusion. The association of chlorhexidine with calcium hydroxide did not increase the antibacterial activity of the intracanal medication in the treatment of primary teeth with necrotic pulp with and without furcal/periapical lesion. (AU)

• Processo FAPESP: 07/01183-0 - Avaliação microbiológica e efeito da pasta de hidróxido de cálcio associada a clorexidina como medicação intracanal no tratamento de dentes decíduos humanos com polpa necrosada
• Beneficiário: Juliana Oliveira Gondim
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 07/54433-4 - Avaliacao microbiologica e efeito da pasta de hidroxido de calcio associada a clorexidina como medicacao intracanal no tratamento de dentes deciduos humanos com polpa necrosada.
• Beneficiário: Elisa Maria Aparecida Giro
• Modalidade de apoio: Auxílio à Pesquisa - Regular