Doxycycline conjugated with polyvinylpyrrolidone-encapsulated silver nanoparticles: a polymer's malevolent touch against Escherichia coli

Autor(es)

Silva, Heloiza F. O. ^[1] ; Lima, Kassio M. G. ^[1] ; Cardoso, Mateus B. ^[2] ; Oliveira, Jessica F. A. ^[2] ; Melo, Maria C. N. ^[3] ; Sant'Anna, Celso ^[4] ; Eugenio, Mateus ^[4] ; Gasparotto, Luiz H. S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Rio Grande do Norte, Inst Chem, Grp Biol Chem & Chemometr, BR-59072970 Natal, RN - Brazil

^[2] LNLS Brazilian Synchrotron Light Lab, BR-13083970 Campinas, SP - Brazil

^[3] Univ Fed Rio Grande do Norte, Ctr Biosci, Lab Med Bacteriol, BR-59078970 Natal, RN - Brazil

^[4] Natl Inst Metrol Qual & Technol Inmetro, Lab Biotechnol Labio, BR-25250020 Duque De Caxias, RJ - Brazil

• Tipo de documento: Artigo Científico
• Fonte: RSC ADVANCES; v. 5, n. 82, p. 66886-66893, 2015.
• Citações Web of Science: 11

Resumo

The emergence of multi-resistant pathogens has necessitated the investigation of new strategies to cope with this ever-increasing threat to public health. In this context, we combined silver nanoparticles (AgNPs) with doxycycline (DO), an antibiotic from the class of tetracyclines, to evaluate the potentiality of this hybrid as a bactericidal agent against E. coli. Polyvinylpyrrolidone (PVP) was used as a stabilizer to prevent the excessive growth and agglomeration of AgNPs. Interestingly, DO bound directly to PVP and had its concentration increased around the particle as a consequence of this interaction. As a result, the AgNPs/DO conjugates presented enhanced bactericidal properties compared to the individual components. Stabilizing agents are generally unwanted on the surfaces of nanoparticles because of their potential to block adsorption surface sites. However, we have shown that PVP played a paramount role in concentrating DO around the particle, which culminated in an increased bactericidal activity towards E. coli. (AU)

• Processo FAPESP: 14/22322-2 - Funcionalização de nanopartículas de sílica: aumentando a interação biológica
• Beneficiário: Mateus Borba Cardoso
• Modalidade de apoio: Auxílio à Pesquisa - Regular