First Molecular Characterization of Cryptosporidium spp. Infecting Buffalo Calves in Brazil

Autor(es)

Aquino, Monally C. C. ^[1] ; Widmer, Giovanni ^[2] ; Zucatto, Anaiza S. ^[1] ; Viol, Milena A. ^[1] ; Inacio, Sandra V. ^[1] ; Nakamura, Alex A. ^[1] ; Coelho, Willian M. D. ^[3] ; Perri, Silvia H. V. ^[1] ; Meireles, Marcelo V. ^[1] ; Bresciani, Katia D. S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Fac Med Vet Aracatuba, Sao Paulo - Brazil

^[2] Tufts Univ, Cummings Sch Vet Med, Dept Infect Dis & Global Hlth, North Grafton, MA - USA

^[3] Univ Estadual Paulista, UNESP, Fac Ciencias Agr & Vet, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Eukaryotic Microbiology; v. 62, n. 5, p. 657-661, SEP-OCT 2015.
• Citações Web of Science: 3

Resumo

With the aim of determining the occurrence of Cryptosporidium spp., 222 fecal samples were collected from Murrah buffalo calves aged up to 6 mo. Fecal DNA was genotyped with a nested polymerase chain reaction targeting the 18S rRNA gene and sequencing of the amplified fragment. Nested 18S PCR was positive for 48.2% of the samples. Sequence analysis showed that the most frequent species in these animals was Cryptosporidium ryanae, which was present in buffalo calves as young as 5 d. The zoonotic species Cryptosporidium parvum was detected in one animal. An uncommon Cryptosporidium 18S genotype was found in buffaloes. (AU)

• Processo FAPESP: 10/52542-3 - Ocorrência da infecção por Cryptosporidium spp. em filhotes de bubalinos, caprinos e ovinos
• Beneficiário: Katia Denise Saraiva Bresciani
• Modalidade de apoio: Auxílio à Pesquisa - Regular