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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

First Molecular Characterization of Cryptosporidium spp. Infecting Buffalo Calves in Brazil

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Author(s):
Aquino, Monally C. C. [1] ; Widmer, Giovanni [2] ; Zucatto, Anaiza S. [1] ; Viol, Milena A. [1] ; Inacio, Sandra V. [1] ; Nakamura, Alex A. [1] ; Coelho, Willian M. D. [3] ; Perri, Silvia H. V. [1] ; Meireles, Marcelo V. [1] ; Bresciani, Katia D. S. [1]
Total Authors: 10
Affiliation:
[1] Univ Estadual Paulista, UNESP, Fac Med Vet Aracatuba, Sao Paulo - Brazil
[2] Tufts Univ, Cummings Sch Vet Med, Dept Infect Dis & Global Hlth, North Grafton, MA - USA
[3] Univ Estadual Paulista, UNESP, Fac Ciencias Agr & Vet, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Eukaryotic Microbiology; v. 62, n. 5, p. 657-661, SEP-OCT 2015.
Web of Science Citations: 3
Abstract

With the aim of determining the occurrence of Cryptosporidium spp., 222 fecal samples were collected from Murrah buffalo calves aged up to 6 mo. Fecal DNA was genotyped with a nested polymerase chain reaction targeting the 18S rRNA gene and sequencing of the amplified fragment. Nested 18S PCR was positive for 48.2% of the samples. Sequence analysis showed that the most frequent species in these animals was Cryptosporidium ryanae, which was present in buffalo calves as young as 5 d. The zoonotic species Cryptosporidium parvum was detected in one animal. An uncommon Cryptosporidium 18S genotype was found in buffaloes. (AU)

FAPESP's process: 10/52542-3 - Occurrence of the infection by Cryptosporidium spp. in water buffaloes, goats and sheeps
Grantee:Katia Denise Saraiva Bresciani
Support Opportunities: Regular Research Grants