Characterization of metabolic profile of intact non-tumor and tumor breast cells by high-resolution magic angle spinning nuclear magnetic resonance spectroscopy

Autor(es)

Maria, Roberta M. ^{[1, 2]} ; Altei, Wanessa F. ^[3] ; Andricopulo, Adriano D. ^[3] ; Becceneri, Amanda B. ^[4] ; Cominetti, Marcia R. ^[4] ; Venancio, Tiago ^[5] ; Colnago, Luiz A. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil

^[2] Embrapa Instrumentacao, BR-13560970 Sao Carlos, SP - Brazil

^[3] Univ Sao Paulo, Inst Fis Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil

^[4] Univ Fed Sao Carlos CCBS UFSCar, Ctr Ciencias Biol & Saude, Dept Gerontol, Lab Biol Envelhecimento LABEN, BR-13565905 Sao Carlos, SP - Brazil

^[5] Univ Fed Sao Carlos, Ctr Ciencias Exatas & Tecnol, Dept Quim, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Analytical Biochemistry; v. 488, p. 14-18, NOV 1 2015.
• Citações Web of Science: 14

Resumo

H-1 high-resolution magic angle spinning nuclear magnetic resonance (H-1 HR MAS NMR) spectroscopy was used to analyze the metabolic profile of an intact non-tumor breast cell line (MCF-10A) and intact breast tumor cell lines (MCF-7 and MDA-MB-231). In the spectra of MCF-10A cells, six metabolites were assigned, with glucose and ethanol in higher concentrations. Fifteen metabolites were assigned in MCF-7 and MDA-MB-231 1H HR MAS NMR spectra. They did not show glucose and ethanol, and the major component in both tumor cells was phosphocholine (higher in MDA-MB-231 than in MCF-7), which can be considered as a tumor biomarker of breast cancer malignant transformation. These tumor cells also show acetone signal that was higher in MDA-MB-231 cells than in MCF-7 cells. The high acetone level may be an indication of high demand for energy in MDA-MB-231 to maintain cell proliferation. The higher acetone and phosphocholine levels in MDA-MB-231 cells indicate the higher malignance of the cell line. Therefore, HR MAS is a rapid reproducible method to study the metabolic profile of intact breast cells, with minimal sample preparation and contamination, which are critical in the analyses of slow-growth cells. (C) 2015 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 09/17846-4 - Análise Metabolômica em linhagens de células tumorais tratadas com ácido linoléico conjugado por Ressonância Magnética Nuclear
• Beneficiário: Roberta Manzano Maria
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/05128-5 - Investigação do perfil metabólico de células de câncer de mama submetidas à quimioterapia através da técnica de ressonância magnética nuclear.
• Beneficiário: Roberta Manzano Maria
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado