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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Characterization of metabolic profile of intact non-tumor and tumor breast cells by high-resolution magic angle spinning nuclear magnetic resonance spectroscopy

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Author(s):
Maria, Roberta M. [1, 2] ; Altei, Wanessa F. [3] ; Andricopulo, Adriano D. [3] ; Becceneri, Amanda B. [4] ; Cominetti, Marcia R. [4] ; Venancio, Tiago [5] ; Colnago, Luiz A. [2]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil
[2] Embrapa Instrumentacao, BR-13560970 Sao Carlos, SP - Brazil
[3] Univ Sao Paulo, Inst Fis Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil
[4] Univ Fed Sao Carlos CCBS UFSCar, Ctr Ciencias Biol & Saude, Dept Gerontol, Lab Biol Envelhecimento LABEN, BR-13565905 Sao Carlos, SP - Brazil
[5] Univ Fed Sao Carlos, Ctr Ciencias Exatas & Tecnol, Dept Quim, BR-13565905 Sao Carlos, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Analytical Biochemistry; v. 488, p. 14-18, NOV 1 2015.
Web of Science Citations: 14
Abstract

H-1 high-resolution magic angle spinning nuclear magnetic resonance (H-1 HR MAS NMR) spectroscopy was used to analyze the metabolic profile of an intact non-tumor breast cell line (MCF-10A) and intact breast tumor cell lines (MCF-7 and MDA-MB-231). In the spectra of MCF-10A cells, six metabolites were assigned, with glucose and ethanol in higher concentrations. Fifteen metabolites were assigned in MCF-7 and MDA-MB-231 1H HR MAS NMR spectra. They did not show glucose and ethanol, and the major component in both tumor cells was phosphocholine (higher in MDA-MB-231 than in MCF-7), which can be considered as a tumor biomarker of breast cancer malignant transformation. These tumor cells also show acetone signal that was higher in MDA-MB-231 cells than in MCF-7 cells. The high acetone level may be an indication of high demand for energy in MDA-MB-231 to maintain cell proliferation. The higher acetone and phosphocholine levels in MDA-MB-231 cells indicate the higher malignance of the cell line. Therefore, HR MAS is a rapid reproducible method to study the metabolic profile of intact breast cells, with minimal sample preparation and contamination, which are critical in the analyses of slow-growth cells. (C) 2015 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 09/17846-4 - Metabolomic analysis in tumor cellular lineages treated with conjugated linoleic acid by Nuclear Magnetic Resonance
Grantee:Roberta Manzano Maria
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 13/05128-5 - Using the technique of nuclear magnetic resonance in investigation of metabolic profile from breast cancer cells subjected to chemotherapy.
Grantee:Roberta Manzano Maria
Support Opportunities: Scholarships in Brazil - Post-Doctoral