Evidence of cAMP involvement in cellobiohydrolase expression and secretion by Trichoderma reesei in presence of the inducer sophorose

Autor(es)

Vieira Nogueira, Karoline Maria ^[1] ; Costa, Mariana do Nascimento ^[1] ; de Paula, Renato Graciano ^[1] ; Mendonca-Natividade, Flavia Costa ^[2] ; Ricci-Azevedo, Rafael ^[2] ; Silva, Roberto Nascimento ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Biochem & Immunol, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Cell Biol & Mol & Pathogen Bioagents, BR-14049900 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BMC Microbiology; v. 15, SEP 30 2015.
• Citações Web of Science: 12

Resumo

Background: The signaling second messenger cyclic AMP (cAMP) regulates many aspects of cellular function in all organisms. Previous studies have suggested a role for cAMP in the regulation of gene expression of cellulolytic enzymes in Trichoderma reesei (anamorph of Hypocrea jecorina). Methods: The effects of cAMP in T. reesei were analyzed through both activity and expression of cellulase, intracellular cAMP level measurement, western blotting, indirect immunofluorescence and confocal microscopy. Results: To elucidate the involvement of cAMP in the cellulase expression, we analyzed the growth of the mutant strain Delta acy1 and its parental strain QM9414 in the presence of the inducers cellulose, cellobiose, lactose, or sophorose, and the repressor glucose. Our results indicated that cAMP regulates the expression of cellulase in a carbon source-dependent manner. The expression cel7a, and cel6a genes was higher in the presence of sophorose than in the presence of cellulose, lactose, cellobiose, or glucose. Moreover, intracellular levels of cAMP were up to four times higher in the presence of sophorose compared to other carbon sources. Concomitantly, our immunofluorescence microscopy and western blot data suggest that in the presence of sophorose, cAMP may regulate secretion of cellulolytic enzymes in T. reesei. Conclusions: These results allow us to better understand the role of cAMP and expand our knowledge on the signal transduction pathways involved in the regulation of cellulase expression in T. reesei. Finally, our data may help develop new strategies to improve the expression of cel7a and cel6a genes, and therefore, favor their application in several biotechnology fields. (AU)

• Processo FAPESP: 14/23653-2 - Caracterização de novos transportadores de açúcar envolvidos na regulação da degradação da biomassa lignocelulósica em Trichoderma reesei
• Beneficiário: Roberto do Nascimento Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/03681-9 - Atuação da caseína-quinase II na repressão catabólica de carbono (CCR) em Trichoderma reesei (Hypocrea jecorina)
• Beneficiário: Karoline Maria Vieira Nogueira
• Modalidade de apoio: Bolsas no Brasil - Mestrado