Neutrophil Extracellular Traps Identification in Tegumentary Lesions of Patients with Paracoccidioidomycosis and Different Patterns of NETs Generation In Vitro

Autor(es)

Della Coletta, Amanda Manoel ^[1] ; Bachiega, Tatiana Fernanda ^[2] ; de Quaglia e Silva, Juliana Carvalho ^[1] ; Victoriano de Campos Soares, Angela Maria ^[2] ; De Faveri, Julio ^[1] ; Marques, Silvio Alencar ^[3] ; Alencar Marques, Mariangela Esther ^[1] ; Ximenes, Valdecir Farias ^[4] ; Dias-Melicio, Luciane Alarcao ^[1]

Afiliação do(s) autor(es)

^[1] UNESP Sao Paulo State Univ, Dept Pathol, Botucatu Med Sch, Botucatu, SP - Brazil

^[2] UNESP Sao Paulo State Univ, Dept Microbiol & Immunol, Biosci Inst, Botucatu, SP - Brazil

^[3] UNESP Sao Paulo State Univ, Dept Dermatol & Radiotherapy, Botucatu Med Sch, Botucatu, SP - Brazil

^[4] UNESP Sao Paulo State Univ, Sch Sci, Dept Chem, Bauru, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS Neglected Tropical Diseases; v. 9, n. 9 SEP 2015.
• Citações Web of Science: 9

Resumo

Paracoccidioidomycosis (PCM) is a systemic mycosis, endemic in most Latin American countries, especially in Brazil. It is caused by the thermo-dimorphic fungus of the genus Paracoccidioides (Paracoccidioides brasiliensis and Paracoccidioides lutzii). Innate immune response plays a crucial role in host defense against fungal infections, and neutrophils (PMNs) are able to combat microorganisms with three different mechanisms: phagocytosis, secretion of granular proteins, which have antimicrobial properties, and the most recent described mechanism called NETosis. This new process is characterized by the release of net-like structures called Neutrophil Extracellular Traps (NETs), which is composed of nuclear (decondensed DNA and histones) and granular material such as elastase. Several microorganisms have the ability of inducing NETs formation, including gram-positive and gram-negative bacteria, viruses and some fungi. We proposed to identify NETs in tegumentary lesions of patients with PCM and to analyze the interaction between two strains of P. brasiliensis and human PMNs by NETs formation in vitro. In this context, the presence of NETs in vivo was evidenced in tegumentary lesions of patients with PCM by confocal spectrum analyzer. Furthermore, we showed that the high virulent P. brasiliensis strain 18 (Pb18) and the lower virulent strain Pb265 are able to induce different patterns of NETs formation in vitro. The quantification of extracellular DNA corroborates the idea of the ability of P. brasiliensis in inducing NETs release. In conclusion, our data show for the first time the identification of NETs in lesions of patients with PCM and demonstrate distinct patterns of NETs in cultures challenged with fungi in vitro. The presence of NETs components both in vivo and in vitro open new possibilities for the detailed investigation of immunity in PCM. (AU)

• Processo FAPESP: 11/18855-7 - Identificação das NETs (neutrophil extracellular traps) em lesões tegumentares de pacientes com paracoccidioidomicose
• Beneficiário: Luciane Alarcão Dias-Melicio
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/00788-7 - Identificação de NETs (neutrophil extracellular traps) em lesões tegumentares de pacientes com paracoccidioidomicose
• Beneficiário: Amanda Manoel Della Coletta
• Modalidade de apoio: Bolsas no Brasil - Mestrado