Isolation and characterization of Saccharomyces species for bioethanol production from sugarcane molasses: Studies of scale up in bioreactor

Autor(es)

Laura Muruaga, Maria ^{[1, 2]} ; Carvalho, Katia G. ^[1] ; Manuel Dominguez, Jose ^[3] ; de Souza Oliveira, Ricardo Pinheiro ^[4] ; Perotti, Nora ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] PROIMI CONICET, San Miguel De Tucuman, Tucuman - Argentina

^[2] Natl Univ Tucuman, San Miguel De Tucuman - Argentina

^[3] Univ Vigo, Fac Sci, Dept Chem Engn, Orense 32004 - Spain

^[4] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Biochem & Pharmaceut Technol, BR-05508900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: RENEWABLE ENERGY; v. 85, p. 649-656, JAN 2016.
• Citações Web of Science: 4

Resumo

Decreases in oil reserves and gas fields around all over the world justify the deepening of studies to render viable the larger-scale use of new energy sources. Therefore, the use of microorganisms to convert sugars into ethanol is a feasible process to be performed in a short period of time and at low costs. In this context, this study aimed to select ethanol-producing yeasts, after isolating samples in molasses obtained from companies in the Province of Tucuman (Argentina) and grapes obtained from farms located in Cafayate (Salta, Argentina). Among the twenty-nine samples studied A2, A10 and All isolates showed higher ethanol productions of 12.87; 13.64 and 13.46% respectively. A2 showed a homogeneous growth meanwhile the growth of strains A10 and All was flocculent. Molecular taxonomic characterization of these isolates showed a percentage of similarity of 100% with the strain Saccharomyces cerevisiae. The behavior of the non-flocculent A2 strain at laboratory scale was faster using a sugarcane molasses based medium, reaching 11.36% ethanol without adding nutrients and other growth factors, probably because its disperse form facilitates the transfer of nutrients and products. These values were improved to 12.02% when the process was scaled up to a 10L bioreactor. All these studies allowed concluding that S. cerevisiae A2 strain is a promising microorganism for the production of bioethanol with potential environmental, energy and economic benefits to be projected into industrial scale. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 13/12713-1 - Produção biotecnológica e purificação de plantaricina por Lactobacillus plantarum St16Pa utilizando meio de cultivo natural
• Beneficiário: Ricardo Pinheiro de Souza Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular