Global performance parameters for different pneumatic bioreactors operating with water and glycerol solution: experimental data and CFD simulation

Autor(es)

Rodriguez, G. Y. ^[1] ; Valverde-Ramirez, M. ^[1] ; Mendes, C. E. ^[1] ; Bettega, R. ^[1] ; Badino, A. C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Grad Program Chem Engn, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Bioprocess and Biosystems Engineering; v. 38, n. 11, p. 2063-2075, NOV 2015.
• Citações Web of Science: 4

Resumo

Global variables play a key role in evaluation of the performance of pneumatic bioreactors and provide criteria to assist in system selection and design. The purpose of this work was to use experimental data and computational fluid dynamics (CFD) simulations to determine the global performance parameters gas holdup ((alpha) over bar (G)) and volumetric oxygen transfer coefficient (k(L)a), and conduct an analysis of liquid circulation velocity, for three different geometries of pneumatic bioreactors: bubble column, concentric-tube airlift, and split tube airlift. All the systems had 5 L working volumes and two Newtonian fluids of different viscosities were used in the experiments: distilled water and 10 cP glycerol solution. Considering the high oxygen demand in certain types of aerobic fermentations, the assays were carried out at high flow rates. In the present study, the performances of three pneumatic bioreactors with different geometries and operating with two different Newtonian fluids were compared. A new CFD modeling procedure was implemented, and the simulation results were compared with the experimental data. The findings indicated that the concentric-tube airlift design was the best choice in terms of both gas holdup and volumetric oxygen transfer coefficient. The CFD results for gas holdup were consistent with the experimental data, and indicated that k (L) a was strongly influenced by bubble diameter and shape. (AU)

• Processo FAPESP: 11/23807-1 - Desenvolvimento e avaliação das condições de operação de biorreatores visando a produção de produtos biotecnológicos
• Beneficiário: Alberto Colli Badino Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/17756-8 - Avaliação das condições hidrodinâmicas, de cisalhamento e de transferência de oxigênio de diferentes modelos de biorreatores pneumáticos
• Beneficiário: Caroline Eliza Mendes
• Modalidade de apoio: Bolsas no Brasil - Doutorado