Amperometric flow-injection determination of the anthelmintic drugs ivermectin and levamisole using electrochemically pretreated boron-doped diamond electrodes

Autor(es)

Lourencao, Bruna C. ^[1] ; Medeiros, Roberta A. ^{[2, 1]} ; Thomasi, Sergio Scherrer ^[1] ; Ferreira, Antonio Gilberto ^[1] ; Rocha-Filho, Romeu C. ^[1] ; Fatibello-Filho, Orlando ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Quim, BR-13560970 Sao Carlos, SP - Brazil

^[2] Univ Estadual Oeste Parana, Ctr Engn & Ciencias Exatas, BR-85903000 Toledo, PR - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SENSORS AND ACTUATORS B-CHEMICAL; v. 222, p. 181-189, JAN 2016.
• Citações Web of Science: 16

Resumo

Sensitive, simple, low-cost, and rapid amperometric flow-injection methods were developed for determination of ivermectin (IVM) and levamisole (LVM) in anthelmintic pharmaceutical drugs and urine samples. These determinations were carried out with a cathodically (for IVM) or anodically (for LVM) pretreated boron-doped diamond electrode using aqueous 0.5 mol L-1 H2SO4 (with and without addition of 30% V/V of ethanol) as supporting electrolyte. The amperometric detection was performed at an applied electrode potential of E = 1.40V, for IVM, and E = 1.90V, for LVM, vs. Ag/AgCl (3.0 mol L-1 KCl). The respective analytical curves presented good linearity in the investigated concentration ranges (0.60-50 mu mol L-1, for IVM, and 0.010-5.0 mu mol L-1; for LVM) and the detection limits were 0.30 mu mol L-1 for IVM, and 1 nmol L-1, for LVM. Investigation of possible interferents showed no significant interference with the here-proposed methods; thus, they were used to determine IVM and LVM in pharmaceutical formulations as well as in urine samples, with results that showed good agreement with comparative methods. Furthermore, a mechanism for LVM electrooxidation was proposed using exhaustive potentiostatic electrolysis and NMR measurements. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 09/17729-8 - Eletrodo de Diamante Dopado com Boro para a Determinação Voltamétrica/ Amperométrica de Anti-helminticos e Anti-hipertensivos
• Beneficiário: Bruna Cláudia Lourenção
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/03019-7 - Avaliação e aplicação analítica de eletrodos de diamante dopado com boro porosos para a determinação voltamétrica das biomoléculas, ácido ascórbico, ácido úrico, dopamina e adrenalina em fluidos biológicos e/ou formulações farmacêuticas
• Beneficiário: Bruna Cláudia Lourenção
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/00601-9 - Aplicação de eletrodos de diamante dopado com boro e de nitreto de carbono amorfo para a determinação simultânea de fármacos de diferentes grupos terapêuticos em formulações farmacêuticas e em urina
• Beneficiário: Roberta Antigo Medeiros
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado