| Texto completo | |
| Autor(es): Mostrar menos - |
Miriam YH Ueda
[1]
;
Paulo G Alvarenga
[2]
;
Juliana M Real
[3]
;
Eloisa de Sá Moreira
[4]
;
Aripuanã Watanabe
[5]
;
Ana Maria Passos-Castilho
[6]
;
Matheus Vescovi
[7]
;
Yana Novis
[8]
;
Vanderson Rocha
[9]
;
Adriana Seber
[10]
;
Jose SR Oliveira
[11]
;
Celso A Rodrigues
;
Celso FH Granato
[13]
Número total de Autores: 13
|
| Afiliação do(s) autor(es): Mostrar menos - | [1] Universidade Federal de São Paulo - Brasil
[2] Universidade Federal de São Paulo - Brasil
[3] Hospital Sírio Libanês. Instituto de Ensino e Pesquisa. Centro de Oncologia - Brasil
[4] Dendrix Research - Brasil
[5] Universidade Federal de São Paulo - Brasil
[6] Universidade Federal de São Paulo - Brasil
[7] Universidade Federal de São Paulo - Brasil
[8] Hospital Sírio Libanês. Instituto de Ensino e Pesquisa. Centro de Oncologia - Brasil
[9] Hospital Sírio Libanês. Instituto de Ensino e Pesquisa. Centro de Oncologia - Brasil
[10] Instituto de Oncologia Pediátrica - Brasil
[11] Universidade Federal de São Paulo - Brasil
[13] Universidade Federal de São Paulo - Brasil
Número total de Afiliações: 13
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Memórias do Instituto Oswaldo Cruz; v. 110, n. 4, p. 461-467, 2015-06-02. |
| Resumo | |
Human herpesvirus 6 (HHV-6) may cause severe complications after haematopoietic stem cell transplantation (HSCT). Monitoring this virus and providing precise, rapid and early diagnosis of related clinical diseases, constitute essential measures to improve outcomes. A prospective survey on the incidence and clinical features of HHV-6 infections after HSCT has not yet been conducted in Brazilian patients and the impact of this infection on HSCT outcome remains unclear. A rapid test based on real-time quantitative polymerase chain reaction (qPCR) has been optimised to screen and quantify clinical samples for HHV-6. The detection step was based on reaction with TaqMan® hydrolysis probes. A set of previously described primers and probes have been tested to evaluate efficiency, sensitivity and reproducibility. The target efficiency range was 91.4% with linearity ranging from 10-106 copies/reaction and a limit of detection of five copies/reaction or 250 copies/mL of plasma. The qPCR assay developed in the present study was simple, rapid and sensitive, allowing the detection of a wide range of HHV-6 loads. In conclusion, this test may be useful as a practical tool to help elucidate the clinical relevance of HHV-6 infection and reactivation in different scenarios and to determine the need for surveillance. (AU) | |
| Processo FAPESP: | 08/57498-2 - Quantificacao simultanea de herpesvirus e seu impacto clinico em pacientes submetidos a transplante de celulas progenitoras hematopoeticas |
| Beneficiário: | Celso Arrais Rodrigues da Silva |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |