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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Isoflurane and Propofol Contribute to Increasing the Antioxidant Status of Patients During Minor Elective Surgery A Randomized Clinical Study

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Autor(es):
Braz, Mariana G. [1, 2] ; Braz, Leandro G. [1] ; Freire, Cristiana M. M. [1] ; Lucio, Lorena M. C. [1] ; Braz, Jose R. C. [1] ; Tang, Guangwen [2] ; Salvadori, Daisy M. F. [1] ; Yeum, Kyung-Jin [2, 3]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista, Fac Med Botucatu, UNESP, BR-18618970 Botucatu, SP - Brazil
[2] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA - USA
[3] Konkuk Univ, Coll Biomed & Hlth Sci, Chungcheongbuk Do - South Korea
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: MEDICINE; v. 94, n. 31 AUG 2015.
Citações Web of Science: 10
Resumo

Isoflurane is a volatile halogenated anesthetic used especially for anesthesia maintenance whereas propofol is a venous anesthetic utilized for anesthesia induction and maintenance, and reportedly an antioxidant. However, there are still controversies related to isoflurane-induced oxidative stress and it remains unanswered whether the antioxidant effects occur in patients under propofol anesthesia.Taking into account the importance of better understanding the role of anesthetics on oxidative stress in anesthetized patients, the present study was designed to evaluate general anesthesia maintained with isoflurane or propofol on antioxidant status in patients who underwent minimally invasive surgeries.We conducted a prospective randomized trial in 30 adult patients without comorbidities who underwent elective minor surgery (septoplasty) lasting at least 2h admitted to a Brazilian tertiary hospital.The patients were randomly allocated into 2 groups, according to anesthesia maintenance (isoflurane, n=15 or propofol, n=15). Peripheral blood samples were drawn before anesthesia (baseline) and 2-h after anesthesia induction.The primary outcomes were to investigate the effect of either isoflurane or propofol anesthesia on aqueous plasma oxidizability and total antioxidant performance (TAP) by fluorometry as well as several individual antioxidants by high-performance liquid chromatography. As secondary outcome, oxidized genetic damage (7,8-dihydro-8-oxoguanine, known as 8-oxo-Gua) was investigated by the comet assay.Both anesthesia techniques (isoflurane or propofol) for a 2-h period resulted in a significant decrease of plasma -tocopherol, but not other antioxidants including uric acid, carotenoids, and retinol (P>0.05). Propofol, in contrast to isoflurane anesthesia, significantly increased (P<0.001) anti-inflammatory/antioxidant plasma -tocopherol concentration in patients. Both anesthesia types significantly enhanced hydrophilic antioxidant capacity and TAP, with no significant difference between them, and 8-oxo-Gua remained unchanged during anesthesia in both groups. In addition, both anesthetics showed antioxidant capacity in vitro.This study shows that anesthesia maintained with either propofol or isoflurane increase both hydrophilic and total antioxidant capacity in plasma, but only propofol anesthesia increases plasma -tocopherol concentration. Additionally, both types of anesthetics do not lead to oxidative DNA damage in patients without comorbidities undergoing minimally invasive surgery. (AU)

Processo FAPESP: 09/17344-9 - Perfil de expressão gênica e estresse oxidativo em pacientes submetidos à cirurgia eletiva sob anestesia geral com propofol ou isoflurano
Beneficiário:Mariana Gobbo Braz
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 10/05611-0 - Perfil de expressão gênica e estresse oxidativo em pacientes submetidos à cirurgia eletiva sob anestesia geral com propofol ou isoflurano
Beneficiário:Daisy Maria Favero Salvadori
Linha de fomento: Auxílio à Pesquisa - Regular