| Texto completo | |
| Autor(es): |
Fernanda da Silveira Vargas
[1]
;
Diana Gabriela Soares
[2]
;
Fernanda Gonçalves Basso
[3]
;
Josimeri Hebling
[4]
;
Carlos Alberto de Souza Costa
[5]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Univ Estadual Paulista - UNESP. Araraquara School of Dentistry. Department of Dental Materials and Prosthodontics - Brasil
[2] Univ Estadual Paulista - UNESP. Araraquara School of Dentistry. Department of Physiology and Pathology - Brasil
[3] Univ Estadual Paulista - UNESP. Araraquara School of Dentistry. Department of Orthodontics and Pediatric Dentistry - Brasil
[4] Univ Estadual Paulista - UNESP. Araraquara School of Dentistry. Department of Orthodontics and Pediatric Dentistry - Brasil
[5] Univ Estadual Paulista - UNESP. Araraquara School of Dentistry. Department of Physiology and Pathology - Brasil
Número total de Afiliações: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Brazilian Dental Journal; v. 25, n. 5, p. 367-371, 2014-10-00. |
| Resumo | |
This in vitro study evaluated the potential protective effect of vitamin E alpha-tocopherol (α-T) isomer against the toxicity of hydrogen peroxide (HP) applied on dental pulp cells. Odontoblast-like MDPC-23 cells were seeded on 96-well plates for 72 h, treated with different concentrations of α-T (1, 3, 5, and 10 mM) for different times (1, 4, 8, and 24 h) and then exposed or not to a 0.018% HP solution for 30 min. In positive and negative control groups, cells were exposed to HP or culture medium (DMEM containing 5% DMSO), respectively. Cell viability was assessed by the MTT assay and the absorbance numeric data, expressed as percentage values, were subjected to the statistical analysis by Kruskal-Wallis and Mann-Whitney tests (α=5%). Considering the cells in the negative control as having 100% of cell viability, all combinations of α-T concentrations and pretreatment times showed a protective effect against HP cytotoxicity. Significant reduction of cell viability (59%) was observed in the positive control compared with the negative control. The highest values of pulp cell viability were obtained after pretreatment with 1 and 3 mM α-T concentrations for 24 h followed by exposure to HP (126% and 97% of cell viability, respectively). Under the tested conditions, the most effective cell protection against the cytotoxic effects of HP was provided by the lowest concentrations of α-T (1 and 3 mM) applied for 24 h. (AU) | |
| Processo FAPESP: | 13/23520-0 - Bioatividade de scaffolds experimentais a base de quitosana e colágeno sobre cultura de células pulpares humanas |
| Beneficiário: | Diana Gabriela Soares dos Passos |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |