Semen characteristics and refrigeration in free-ranging giant anteaters (Myrmecophaga tridactyla)

Autor(es)

Luba, Camila do Nascimento ^[1] ; Boakari, Yatta Linhares ^[1] ; Costa Lopes, Alexandre Martins ^[2] ; Gomes, Marcelo da Silva ^[2] ; Miranda, Flavia Regina ^[2] ; Papa, Frederico Ozanan ^[1] ; Pinheiro Ferreira, Joao Carlos ^[1]

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Sch Vet Med & Anim Sci, Dept Anim Reprod & Vet Radiol, Botucatu, SP - Brazil

^[2] Brazilian Anteaters Res & Conservat Inst, Projeto Tamandua, Curitiba, Parana - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Theriogenology; v. 84, n. 9, p. 1572-1580, DEC 2015.
• Citações Web of Science: 1

Resumo

The giant anteater (Myrmecophaga tridactyla) is considered vulnerable to extinction. Scientific data on the reproductive parameters of this species are scarce. Semen from eight free-ranging giant anteaters was collected to establish its characteristics and the effects of cooling and storage at 5 degrees C after dilution with the BotuCrio extender without cryoprotectant. The ejaculate presented two distinct sequential fractions, including a whitish fraction, which was milky and rich in sperm cells, and a gel fraction, which was colorless, viscous, and azoospermic. The mean standard error of the mean values of the seminal characteristics were as follows: volume of the first fraction, 0.75 +/- 0.1 mL; motility, 75 +/- 2.9%; vigor, 3.2 +/- 0.3; sperm motility index, 68.8 +/- 4.3; concentration, 108.5 +/- 13.4 x 10(6)/mL; plasma membrane integrity index, 71 +/- 4.0%; spermatic defects detected using modified Karras staining, 35.5 +/- 3.3%; and spermatic alterations identified by differential interference contrast microscopy, 48.3 +/- 6.8%. During refrigeration, the semen presented decreasing motility from 0 to 18 hours, sperm motility index decreased from 0 to 24 hours, and vigor did not change in the first 6 hours and then decreased to 18 hours. (C) 2015 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 12/24538-7 - Características seminais e resfriamento de sêmen de tamanduás-bandeira (Myrmecophaga tridactyla) de vida livre
• Beneficiário: João Carlos Pinheiro Ferreira
• Modalidade de apoio: Auxílio à Pesquisa - Regular