Tooth Tissue Engineering: The Importance of Blood Products as a Supplement in Tissue Culture Medium for Human Pulp Dental Stem Cells

Autor(es)

Pisciolaro, Ricardo Luiz ^{[1, 2]} ; Duailibi, Monica Talarico ^{[1, 2, 3]} ; Novo, Neil Ferreira ^{[2, 4]} ; Juliano, Yara ^{[2, 5, 4]} ; Pallos, Debora ^[5] ; Yelick, Pamela Crotty ^[6] ; Vacanti, Joseph Phillip ^{[7, 8]} ; Ferreira, Lydia Masako ^[2] ; Duailibi, Silvio Eduardo ^{[1, 2, 3]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Ctr Cellular & Mol Therapy, CTCMol, BR-04044010 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Dept Surg, Translat Surg, BR-04044010 Sao Paulo - Brazil

^[3] BIOFABRIS, Natl Inst Sci & Technol, Biofabricat Inst, Campinas, SP - Brazil

^[4] Univ Santo Amaro, Dept Hlth Sci, Sao Paulo - Brazil

^[5] Univ Santo Amaro, Dept Dent, Sao Paulo - Brazil

^[6] Tufts Univ, Dept Oral & Maxillofacial Pathol, Boston, MA 02111 - USA

^[7] Harvard Univ, Sch Med, Boston, MA - USA

^[8] Massachusetts Gen Hosp, Lab Tissue Engn & Organ Fabricat, Boston, MA 02114 - USA

• Tipo de documento: Artigo Científico
• Fonte: TISSUE ENGINEERING PART A; v. 21, n. 21-22, p. 2639-2648, NOV 1 2015.
• Citações Web of Science: 3

Resumo

One of the goals in using cells for tissue engineering (TE) and cell therapy consists of optimizing the medium for cell culture. The present study compares three different blood product supplements for improved cell proliferation and protection against DNA damage in cultured human dental pulp stem cells for tooth TE applications. Human cells from dental pulp were first characterized as adult stem cells (ectomesenchymal mixed origin) by flow cytometry. Next, four different cell culture conditions were tested: I, supplement-free; II, supplemented with fetal bovine serum; III, allogeneic human serum; and IV, autologous human serum. Cultured cells were then characterized for cell proliferation, mineralized nodule formation, and colony-forming units (CFU) capability. After 28 days in culture, the comet assay was performed to assess possible damage in cellular DNA. Our results revealed that Protocol IV achieved higher cell proliferation than Protocol I (p=0.0112). Protocols II and III resulted in higher cell proliferation than Protocol I, but no statistical differences were found relative to Protocol IV. The comet assay revealed less cell damage in cells cultured using Protocol IV as compared to Protocols II and III. The damage percentage observed on Protocol II was significantly higher than all other protocols. CFUs capability was highest using Protocol IV (p=0.0018) and III, respectively, and the highest degree of mineralization was observed using Protocol IV as compared to Protocols II and III. Protocol IV resulted in significantly improved cell proliferation, and no cell damage was observed. These results demonstrate that human blood product supplements can be used as feasible supplements for culturing adult human dental stem cells. (AU)

• Processo FAPESP: 08/57860-3 - BIOFABRIS - Instituto de Biofabricação
• Beneficiário: Rubens Maciel Filho
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 07/51227-4 - Caracterização das células-tronco progenitoras de germes dentais humanos para a utilização em engenharia tecidual dos tecidos mineralizados, osso, cartilagem e dentes
• Beneficiário: Silvio Eduardo Duailibi
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 07/58856-7 - Criopreservacao de dentes humanos para a utilizacao de celulas tronco progenitoras dentais na construcao de substitutos biologicos.
• Beneficiário: Monica Talarico Duailibi
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 07/59488-1 - Caracterizacao das celulas-tronco progenitoras de germes dentais humanos para a utilizacao em engenharia tecidual dos tecidos mineralizados, osso, cartilagem e dentes.
• Beneficiário: Silvio Eduardo Duailibi
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores