Smoking Modulates Gene Expression of Type I Collagen, Bone Sialoprotein, and Osteocalcin in Human Alveolar Bone

Autor(es)

de Campos, Juliana Medeiros ^[1] ; Prati, Alexandre Javaroni ^[1] ; Cirano, Fabiano Ribeiro ^[2] ; Pimentel, Suzana Peres ^[2] ; Pastore, Gabriel Pires ^[1] ; Pecorari, Vanessa Gallego ^[3] ; Ribeiro, Fernanda Vieira ^[2] ; Casati, Marcio Zaffalon ^[2] ; Viana Casarin, Renato Correa ^[2]

Afiliação do(s) autor(es)

^[1] Univ Paulista, Div Maxillofacial Surg, Sao Paulo - Brazil

^[2] Univ Paulista, Div Periodont, Sao Paulo - Brazil

^[3] Univ Paulista, Div Stat, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY; v. 73, n. 11, p. 2123-2131, NOV 2015.
• Citações Web of Science: 13

Resumo

Purpose: Previous animal studies have shown the negative impact of smoking on bone-to-implant contact, and in humans, a decrease in bone density and implant survival over time. However, the effect of smoking on the human alveolar bone regarding the expression of bone-related markers is unknown. Therefore, the aim of this study was to evaluate the influence of smoking on the gene expression of molecules of bone metabolism in alveolar bone tissue from sites designed to receive dental implants. Materials and Methods: Biopsy specimens of alveolar bone were collected from smokers (n = 19) and nonsmokers (n = 19) from areas planned to receive dental implants. Gene expression of tumor necrosis factor (TNF)-alpha, transforming growth factor (TGF)-beta, osteoprotegerin (OPG), type I collagen (COL-I), bone sialoprotein (BSP), and osteocalcin (OCN) was quantified by quantitative real-time polymerase chain reaction using glyceraldehyde-3-phosphate dehydrogenase as a reference gene. The results were assessed using multiple regression analysis, with a significance level of 5%. Results: Multiple regression analysis indicated that smoking negatively affected mRNA expression of BSP and OCN and positively altered the expression of COL-I (P < .05) despite age, gender, and arch. Moreover, regression analysis did not show a significant correlation between smoking habit and mRNA levels of TNF-alpha, TGF-beta, and OPG (P>.05). Conclusion: These results support the hypothesis that some bone markers in alveolar tissue are modulated by smoking, which could explain the negative impact of smoking on bone healing. (C) 2015 American Association of Oral and Maxillofacial Surgeons (AU)

• Processo FAPESP: 13/21977-2 - Reserva técnica para infraestrutura institucional de pesquisa
• Beneficiário: Fernanda Vieira Ribeiro
• Modalidade de apoio: Auxílio à Pesquisa - Reserva Técnica para Infraestrutura Institucional de Pesquisa