Preliminary evidence of age-dependent clinical signs associated with porcine circovirus 2b in experimentally infected CH3/Rockefeller mice

Autor(es):Mostrar menos -

de Castro, Alessandra M. M. G. ^{[1, 2]} ; Cruz, Tais F. ^{[3, 4]} ; Yamada, Katarina B. ^[1] ; Gerber, Priscilla F. ^[5] ; Gabardo, Michelle P. ^[5] ; Araujo, Jr., Joao P. ^{[3, 4]} ; Guedes, Roberto M. C. ^[5] ; Mori, Cinthia K. ^[1] ; Oliveira, Camila P. ^[1] ; Santos, Sueli S. ^[1] ; Richtzenhain, Leonardo J. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Med Vet Prevent & Saude Anim, BR-05508270 Sao Paulo, SP - Brazil

^[2] Complexo Educ Fac Metropolitana Unidas, Vet, Sao Paulo, SP - Brazil

^[3] Univ Estadual Paulista, Inst Biociencia, Dept Imunol & Microbiol, Botucatu, SP - Brazil

^[4] Sao Paulo State Univ UNESP, Biotechnol Inst IBTEC, Sao Paulo, SP - Brazil

^[5] Univ Fed Minas Gerais, Escola Vet, Dept Clin & Cirurgia Vet, BR-31270901 Belo Horizonte, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Research in Veterinary Science; v. 103, p. 70-72, DEC 2015.
• Citações Web of Science: 0

Resumo

Mice and rats are susceptible to porcine circovirus 2b (PCV2) infection under field and experimental conditions. However, whether PCV2 induces disease in rodents remains a matter of debate. The objectives of the present study were to determine whether PCV2-induced disease in mice is age-dependent and whether intranasally inoculated animals are able to infect animals they come into contact with. Twenty-five CH3/Rockefeller mice were divided into six groups and intranasally inoculated with 25 mu L of either PCV2b or PBS on days 0, 3 and 6. One group remained untreated. Two age groups were tested: 3-week-old mice and 6-week-old mice. The administration of three PCV2 intranasal inoculations at intervals of three days was able to induce infection and support virus transmission in susceptible mice, regardless of the age at inoculation. The clinical signs associated with PCV2 infection were more severe in younger mice, and PCV2-DNA load was higher in their faeces. In conclusion, PCV2 induced disease in mice. (C) 2015 Published by Elsevier Ltd. (AU)

• Processo FAPESP: 11/00054-8 - Inoculação experimental de pcv2 em camundongos ch3/ rockfeller aos 21 dias de idade: distribuição viral e dinâmica de eliminação pelas fezes.
• Beneficiário: Katarina Baumgartner Yamada
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 07/57115-3 - Circovirus suíno tipo 2 (PCV-2): patogenia comparada dos subtipos A e B e efeitos sobre o desempenho zootécnico de suínos experimentalmente infectados com amostras brasileiras
• Beneficiário: Alessandra Marnie Martins Gomes de Castro
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 09/09175-2 - Circovírus suíno tipo 2 (PCV-2): patogenia comparada dos subtipos A e B e efeitos sobre o desempenho zootécnico de suínos experimentalmente infectados com amostras brasileiras
• Beneficiário: Alessandra Marnie Martins Gomes de Castro
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores