Immunolocalization of dentin matrix protein-1 in human primary teeth treated with different pulp capping materials

Autor(es)

Lourenco Neto, Natalino ^[1] ; Marques, Nadia C. T. ^[1] ; Fernandes, Ana Paula ^[1] ; Rodini, Camila O. ^[2] ; Sakai, Vivien T. ^[3] ; Abdo, Ruy Cesar C. ^[1] ; Machado, Maria Aparecida A. M. ^[1] ; Santos, Carlos F. ^[2] ; Oliveira, Thais M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Pediat Dent Orthodont & Publ Hlth, Bauru, SP - Brazil

^[2] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru, SP - Brazil

^[3] Univ Fed Alfenas, Dept Clin & Surg, Alfenas, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS; v. 104, n. 1, p. 165-169, JAN 2016.
• Citações Web of Science: 8

Resumo

The aim of this study was to evaluate the immunolocalization of dentin matrix protein (DMP)-1 in human primary teeth treated with different pulp capping materials. Twenty-five primary molars were divided into the following groups: formocresol (FC), calcium hydroxide (CH), mineral trioxide aggregate (MTA), corticosteroid/antibiotic solution+CH (O+CH), and Portland cement (PC), and all received conventional pulpotomy treatment. The teeth at the regular exfoliation period were extracted for histological analysis and immunolocalization of DMP-1. Statistical analysis was performed using the (2) test (p<0.05). Histological analysis revealed statistically significant differences in the comparison among the groups through the use of a score system regarding the presence of hard tissue barrier, odontoblastic layer, and internal resorption, but not regarding pulp calcification. Immunohistochemical analysis showed immunostaining for DMP-1 in groups CH, MTA, O+CH, and PC. Internal resorption was observed in the groups FC and CH. MTA and PC showed pulp repair without inflammation and with the presence of hard tissue barrier. DMP-1 immunostaining was higher for MTA and PC, confirming the reparative and bioinductive capacity of these materials. (c) 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 165-169, 2016. (AU)

• Processo FAPESP: 09/11284-4 - Fator de crescimento endotelial vascular na reposta pulpar após o uso de material capeador precedido por anti-inflamatório ou laser de baixa intensidade em pulpotomias de dentes decíduos humanos
• Beneficiário: Thais Marchini de Oliveira Valarelli
• Modalidade de apoio: Auxílio à Pesquisa - Regular