Exercise reverses OVA-induced inhibition of glucocorticoid receptor and increases anti-inflammatory cytokines in asthma

Autor(es)

Silva, R. A. ^{[1, 2]} ; Almeida, F. M. ^[1] ; Olivo, C. R. ^[1] ; Saraiva-Romanholo, B. M. ^{[1, 3]} ; Martins, M. A. ^[1] ; Carvalho, C. R. F. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med, Dept Clin Med LIM 20, BR-01246903 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Sch Med, Dept Phys Therapy, BR-01246903 Sao Paulo, SP - Brazil

^[3] Univ City Sao Paulo UNICID, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCANDINAVIAN JOURNAL OF MEDICINE & SCIENCE IN SPORTS; v. 26, n. 1, p. 82-92, JAN 2016.
• Citações Web of Science: 5

Resumo

The purpose of this study was to determine the effect of aerobic exercise training (AT) on the expression of glucocorticoid receptors (GR) and anti-inflammatory cytokines in an asthma model. BALB/c mice were divided into groups control (CT; nonsensitized/nontrained), aerobic training (AT; nonsensitized/trained), ovalbumin (OVA; sensitized/not trained), and OVA+AT (sensitized/trained). OVA groups received OVA by inhalation, and the AT groups completed 1, 3, or 7 days of exercise (60min/session). Expression of GR, IL-4, IL-5, IL-10, IL-1ra, NF-B, TGF-, VEGF, ICAM-1, VCAM-1; eosinophils counting; and airway remodeling (AR) features {[}airway smooth muscle (ASM) and epithelial thickness and collagen fiber deposition] were quantified. OVA sensitization induced a decrease in the expression of GR and increases in the eosinophil, IL-4, IL-5, NF-B, TGF-, VEGF, ICAM-1, VCAM-1, and AR features (P<0.05). After 3 days, AT reversed the OVA-induced reduction in the expression of GR, and subsequently induced increases in the expression of IL-10 and IL-1ra (seventh day). In contrast, the eosinophil migration, the expression of NF-B, IL-4, IL-5, TGF-, RANTES, VEGF, ICAM-1, VCAM-1, and the AR features (P<0.05) were reduced. AT increases the expression of GR and anti-inflammatory cytokines (IL-10 and IL-1ra) and reduces the expression of inflammatory mediators and airway inflammation in an animal model of asthma. (AU)

• Processo FAPESP: 09/54420-5 - Estudo dos mecanismos induzidos pelo treinamento físico aeróbico ao longo do tempo na inflamação pulmonar e no remodelamento brônquico em um modelo murino de asma
• Beneficiário: Ronaldo Aparecido da Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 09/53904-9 - Efeitos do ambiente e do estilo de vida sobre a asma e a doença pulmonar obstrutiva crônica: estudos experimentais e clínicos
• Beneficiário: Milton de Arruda Martins
• Modalidade de apoio: Auxílio à Pesquisa - Temático