Iron-regulated proteins in Phanerochaete chrysosporium and Lentinula edodes: Differential analysis by sodium dodecyl sulfate polyacrylamide gel electrophoresis and two-dimensional polyacrylamide gel electrophoresis profiles

Autor(es)

Hernandez-Macedo, ML ; Ferraz, A ; Rodriguez, J ; Ottoboni, LMM ; De Mello, MP

• Tipo de documento: Artigo Científico
• Fonte: ELECTROPHORESIS; v. 23, n. 4, p. 655-661, FEB 2002.
• Citações Web of Science: 22

Resumo

Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional gel electrophoresis (2-DE) were used to identify iron-responsive proteins in the white-rot species (Phanerochaete chrysosporium and Lentinula edodes), by comparing the differential patterns of cellular and membrane proteins obtained from iron-sufficient and iron-deficient mycelia. Six cellular proteins induced by iron restriction have been observed in SDS-PAGE for P. chrysosporium and twelve for L. edodes. In 2-DE, the numbers of iron-restricted induced proteins were 12 and 9, respectively, in a resolution range of 15-60 kDa and pl 4.5-8.1. SDS-PAGE for the plasma membrane protein did not show differences, whereas the outer-membrane protein profile showed 6 and 5 proteins induced by iron depletion in R chrysosporium and L. edodes, respectively. The results presented here are important data to unravel mechanisms of biosynthesis and/or transport of the iron-complexing agents in ligninolytic fungi and to further correlate them to the ligninolytic processes. (AU)

• Processo FAPESP: 98/00548-4 - Identificacao de genes e proteinas envolvidos na producao de sideroforos por fungos que degradam madeira.
• Beneficiário: Maricilda Palandi de Mello
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 98/04519-9 - Identificação de proteínas envolvidas na produção de sideróforos por fungos que degradam madeira
• Beneficiário: Maria Lucila Hernández Macedo
• Modalidade de apoio: Bolsas no Brasil - Mestrado