Experimental evidence and molecular modeling of the interaction between hRSV-NS1 and quercetin

Autor(es)

Gomes, Deriane Elias ^{[1, 2]} ; Caruso, Icaro Putinhon ^{[1, 2]} ; de Araujo, Gabriela Campos ^{[1, 2]} ; de Lourenco, Isabella Otenio ^{[1, 2]} ; de Melo, Fernando Alves ^{[1, 2]} ; Cornelio, Marinonio Lopes ^{[1, 2]} ; Fossey, Marcelo Andres ^{[1, 2]} ; de Souza, Fatima Pereira ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista Julio de Mesquita Filho UN, Dept Fis, Inst Biociencias Letras & Ciencias Exatas IBILCE, Campus Sao Jose de Rio Preto SP, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

^[2] Univ Estadual Paulista Julio de Mesquita Filho UN, CMIB, Inst Biociencias Letras & Ciencias Exatas IBILCE, Campus Sao Jose de Rio Preto SP, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Biological Macromolecules; v. 85, p. 40-47, APR 2016.
• Citações Web of Science: 6

Resumo

Human Respiratory Syncytial Virus is one of the major causes of acute respiratory infections in children, causing bronchiolitis and pneumonia. Non-Structural Protein 1 (NS1) is involved in immune system evasion, a process that contributes to the success of hRSV replication. This protein can act by inhibiting or neutralizing several steps of interferon pathway, as well as by silencing the hRSV ribonucleoproteic complex. There is evidence that quercetin can reduce the infection and/or replication of several viruses, including RSV. The aims of this study include the expression and purification of the NS1 protein besides experimental and computational assays of the NS1-quercetin interaction. CD analysis showed that NS1 secondary structure composition is 30% alpha-helix, 21% beta-sheet, 23% turn and 26% random coils. The melting temperature obtained through DSC analysis was around 56 degrees C. FRET analysis showed a distance of approximately 19 A between the NS1 and quercetin. Fluorescence titration results showed that the dissociation constant of the NS1-quercetin interaction was around 10(-6) M. In thermodynamic analysis, the enthalpy and entropy balanced forces indicated that the NS1-quercetin interaction presented both hydrophobic and electrostatic contributions. The computational results from the molecular modeling for NS1 structure and molecular docking regarding its interaction with quercetin corroborate the experimental data. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 10/50211-0 - Investigação das interações entre ns1 de hRSV e flavonoides para busca de potenciais inibidores
• Beneficiário: Deriane Elias Gomes
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/52266-6 - Estudo das interações proteína-proteína entre NS1 de Vírus Sincicial Respiratório Humano e proteínas celulares e virais: implicações para resposta imunológica e infectividade
• Beneficiário: Fátima Pereira de Souza
• Modalidade de apoio: Auxílio à Pesquisa - Regular