Periodontal Disease Decreases Insulin Sensitivity and Insulin Signaling

Autor(es)

Colombo, Natalia H. ^[1] ; Shirakashi, Daisy J. ^[1] ; Chiba, Fernando Y. ^[2] ; de Lima Coutinho, Maria Sara ^[1] ; Ervolino, Edilson ^[1] ; Saliba Garbin, Clea Adas ^[2] ; Machado, Ubiratan Fabres ^[3] ; Sumida, Doris H. ^[1]

Afiliação do(s) autor(es)

^[1] UNESP Sao Paulo State Univ, Dent Sch Aracatuba, Univ Estadual Paulista, Dept Basic Sci, BR-16015050 Aracatuba, SP - Brazil

^[2] UNESP Sao Paulo State Univ, Dent Sch Aracatuba, Dept Child & Social Dent, BR-16015050 Aracatuba, SP - Brazil

^[3] UNESP Sao Paulo State Univ, Inst Biomed Sci, Dept Physiol & Biophys, BR-16015050 Aracatuba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Periodontology; v. 83, n. 7, p. 864-870, JUL 2012.
• Citações Web of Science: 20

Resumo

Background: The purpose of this study is to investigate whether local inflammatory events, such as periodontal disease, are able to increase tumor necrosis factor-alpha (TNF-alpha) plasmatic concentration and decrease insulin sensitivity and insulin signaling in non-diabetic rats. Methods: Forty-eight male Wistar rats (2 months old) were divided into two groups, with either ligature-induced periodontal disease (LPD) or control conditions (CN). Experiments were performed in both groups 28 days after ligature placement. Plasmatic concentration of glycemia and TNF-alpha (n = 10) were analyzed by the glucose oxidase and enzyme-linked immunosorbent assay method, respectively. Insulin sensitivity (n = 7) was measured using the insulin tolerance test. Insulin signal transduction (n = 7) was measured by pp185 tyrosine phosphorylation status in insulin-sensitive tissues using the Western blotting method. Results: The LPD group showed decreased insulin sensitivity (P <0.05), although no glycemic alterations were noted (P >0.05). TNF-alpha plasmatic concentration was higher in LPD rats compared to CN rats. In addition, a decrease in the pp185 tyrosine phosphorylation status was observed after insulin stimulus in both white adipose and skeletal muscle tissues of the LPD group compared with the CN group. Conclusions: LPD is able to cause alterations to both insulin signaling and insulin sensitivity, probably because of the elevation of TNF-alpha plasmatic concentration. Thus, the present results emphasize the importance of the prevention of local inflammatory diseases, such as periodontitis, to prevent diabetes mellitus. J Periodontot 2012;83:864-870. (AU)

• Processo FAPESP: 09/02976-0 - Avaliação da sensibilidade à insulina em ratos adultos com doença periodontal
• Beneficiário: Natalia Helena Colombo
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 09/02846-9 - Avaliação da transdução do sinal insulínico em ratos adultos com doença periodontal
• Beneficiário: Daisy Jaqueline Shirakashi
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica