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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Periodontal Disease Decreases Insulin Sensitivity and Insulin Signaling

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Author(s):
Colombo, Natalia H. [1] ; Shirakashi, Daisy J. [1] ; Chiba, Fernando Y. [2] ; de Lima Coutinho, Maria Sara [1] ; Ervolino, Edilson [1] ; Saliba Garbin, Clea Adas [2] ; Machado, Ubiratan Fabres [3] ; Sumida, Doris H. [1]
Total Authors: 8
Affiliation:
[1] UNESP Sao Paulo State Univ, Dent Sch Aracatuba, Univ Estadual Paulista, Dept Basic Sci, BR-16015050 Aracatuba, SP - Brazil
[2] UNESP Sao Paulo State Univ, Dent Sch Aracatuba, Dept Child & Social Dent, BR-16015050 Aracatuba, SP - Brazil
[3] UNESP Sao Paulo State Univ, Inst Biomed Sci, Dept Physiol & Biophys, BR-16015050 Aracatuba, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Periodontology; v. 83, n. 7, p. 864-870, JUL 2012.
Web of Science Citations: 20
Abstract

Background: The purpose of this study is to investigate whether local inflammatory events, such as periodontal disease, are able to increase tumor necrosis factor-alpha (TNF-alpha) plasmatic concentration and decrease insulin sensitivity and insulin signaling in non-diabetic rats. Methods: Forty-eight male Wistar rats (2 months old) were divided into two groups, with either ligature-induced periodontal disease (LPD) or control conditions (CN). Experiments were performed in both groups 28 days after ligature placement. Plasmatic concentration of glycemia and TNF-alpha (n = 10) were analyzed by the glucose oxidase and enzyme-linked immunosorbent assay method, respectively. Insulin sensitivity (n = 7) was measured using the insulin tolerance test. Insulin signal transduction (n = 7) was measured by pp185 tyrosine phosphorylation status in insulin-sensitive tissues using the Western blotting method. Results: The LPD group showed decreased insulin sensitivity (P <0.05), although no glycemic alterations were noted (P >0.05). TNF-alpha plasmatic concentration was higher in LPD rats compared to CN rats. In addition, a decrease in the pp185 tyrosine phosphorylation status was observed after insulin stimulus in both white adipose and skeletal muscle tissues of the LPD group compared with the CN group. Conclusions: LPD is able to cause alterations to both insulin signaling and insulin sensitivity, probably because of the elevation of TNF-alpha plasmatic concentration. Thus, the present results emphasize the importance of the prevention of local inflammatory diseases, such as periodontitis, to prevent diabetes mellitus. J Periodontot 2012;83:864-870. (AU)

FAPESP's process: 09/02976-0 - Evaluation of insulin sensitivity in adult rats with periodontal disease.
Grantee:Natalia Helena Colombo
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 09/02846-9 - Assessment of early steps of insulin action in adult rats with periodontal disease
Grantee:Daisy Jaqueline Shirakashi
Support Opportunities: Scholarships in Brazil - Scientific Initiation