Effect of physical activity on insulin receptor substrate phosphorylation status in liver of rats with periodontal disease

• Grant number: 14/01964-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): April 01, 2014
• Effective date (End): March 31, 2015
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Doris Hissako Matsushita
• Grantee: Lara Mariano Pinheiro
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Studies have shown that there is a correlation between Diabetes Mellitus (DM) and Periodontal Disease (PD) so that people with DM present higher prevalence and severity of PD, when compared with healthy people. It is also observed that among people with PD there is an increased prevalence of Diabetes. Previous studies in our laboratory showed that DP induces changes in insulin signaling and insulin resistence. Several studies showed that exercise can promote improvements in insulin sensitivity. Knowing that DP reduces insulin sensitivity and signaling, this study will investigate if physical exercise can revert or improve this condition in rats with PD. Therefore, it will be assessed in rats with PD submitted or not to exercise insulin receptor substrate pp185 (IRS-1/IRS-2) tyrosine phosphorylation status in liver. Therefore, 24 male Wistar rats (at two months old) will be used, and they will be separated in four groups (n=6): 1) sedentary control group without DP (CNS); 2) exercised control group without DP (CNEx); 3) sedentary group with DP (DPS); 4) exercised group with DP (DPEx). In those groups with DP, the disease will be induced by ligating silk thread on the lower first molars. After that, it will be assessed, three times a week, food ingestion and, once a week, body weight of rats in all groups. In the 28th day after the disease induction, the resisted training will begin. After twelve weeks of training, the experiments will be realized. Statistical analysis will be performed by analysis of variance (ANOVA) followed by Tukey's test. Significance value adopted will be of 5%.