A case-control study of CYP2E1 (PstI) and CYP1A1 (MspI) polymorphisms in colorectal cancer

Autor(es)

Proenca, M. A. ^{[1, 2]} ; Fernandes, G. M. M. ^[2] ; Russo, A. ^[2] ; Lelis, R. B. ^[2] ; Netinho, J. G. ^{[3, 4]} ; Cunrath, G. S. ^{[3, 4]} ; Silva, A. E. ^[1] ; Goloni-Bertollo, E. M. ^[2] ; Pavarino, E. C. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Dept Biol, Sao Jose Do Rio Preto, SP - Brazil

^[2] Fac Med Sao Jose do Rio Preto, Dept Biol Mol, Unidade Pesquisa Genet & Biol Mol, Sao Jose Do Rio Preto, SP - Brazil

^[3] Fac Med Sao Jose do Rio Preto, Dept Cirurgia, Sao Jose Do Rio Preto, SP - Brazil

^[4] Fac Med Sao Jose do Rio Preto, Serv Ambulatorial Coloproctol, Sao Jose Do Rio Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Genetics and Molecular Research; v. 14, n. 4, p. 17856-17863, 2015.
• Citações Web of Science: 1

Resumo

Polymorphisms in genes encoding P450 cytochrome enzymes may increase the risk of sporadic colorectal cancer (SCRC). Here we investigated the association between SCRC and CYP2E1 (PstI) and CYP1A1 (MspI) polymorphisms in a case-control study. Moreover, we sought to determine any possible associations between this disease and the sociodemographic factors. We included 273 individuals (74 patients and 199 controls); the gender, age, tobacco usage, and alcohol consumption of the included subjects, and the clinico-histopathological parameters of the tumors, were analyzed. Molecular analyses were performed using PCR-RFLP. The effect of polymorphisms on SCRC development, and the association between this disease and sociodemographic factors were determined by multiple-logistic regression analyses. The combined genotype was also evaluated. Statistically significant differences between the patients and controls regarding the male gender (odds ratio, OR = 0.19, 95% confidence interval, CI = 0.08-0.46; P <= 0.05) and age >= 44 years (median = 44; OR = 96.84, 95% CI = 21.78-430.49; P <= 0.05) were observed. The evaluated polymorphisms were not associated with SCRC (PstI-CYP2E1: OR = 0.93, 95% CI = 0.30-2.85; P = 0.897; MspI-CYP1A1: OR = 0.75, 95% CI = 0.35-1.61; P = 0.463); the combined genotypes were not associated with the risk of disease. Thus, individuals aged >= 44 years are more sensitive to SCRC, while men are less susceptible. Additionally, polymorphisms in CYP2E1 (PstI) and CYP1A1 (MspI) were not associated with SCRC in the evaluated Brazilian population. (AU)

• Processo FAPESP: 12/00130-9 - Análise molecular e epidemiológica dos polimorfismos CYP1A1*2C e CYP2E1*6 em pacientes com câncer colorretal
• Beneficiário: Roliana Bravo Lelis
• Linha de fomento: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 12/02473-0 - Investigação molecular e epidemiológica de genes do metabolismo de xenobióticos em pacientes com câncer colorretal esporádico
• Beneficiário: Glaucia Maria de Mendonça Fernandes
• Linha de fomento: Bolsas no Brasil - Mestrado
• Processo FAPESP: 11/23969-1 - Investigação molecular e epidemiológica de genes do metabolismo de xenobióticos em pacientes com câncer colorretal esporádico
• Beneficiário: Eny Maria Goloni Bertollo
• Linha de fomento: Auxílio à Pesquisa - Regular