Optimization of artificial neural network by genetic algorithm for describing viral production from uniform design data

Autor(es)

Takahashi, Maria Beatriz ^[1] ; Rocha, Jose Celso ^[1] ; Fernandez Nunez, Eutimio Gustavo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Julio de Mesquita Filho Campus Assi, Dept Ciencias Biol, Ave Dom Antonio 2100, BR-19806900 Assis, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Process Biochemistry; v. 51, n. 3, p. 422-430, MAR 2016.
• Citações Web of Science: 8

Resumo

This work objective was to define a modeling approach based on genetic algorithm (GA) for optimizing parameters of an artificial neural network (ANN); the latter describes rabies virus production in BHK-21 cells based on empirical data derived from uniform designs (UDs) with different numbers of experimental runs. The parameters considered for viral infection were temperature (34 and 37 degrees C), multiplicity of infection (0.04, 0.07, and 0.1), infection, and harvest times (24, 48, and 72 h), with virus production as the monitored output variable. A multilevel factorial experimental design was performed and used to train, validate, and test the ANN. Its experimental fractions (18, 24, 30, 36, and 42 runs) defined by UDs were used to simulate the neural architectures. In GA, the neural computing parameters constituted the population individuals, and the steps involved were population creation, selection, and replacement by crossover and mutation. The ANN optimized by the combined algorithm showed a good calibration for all UDs under consideration, thus demonstrating to be suitable (R>0.85) as a correlation method in UDs independent of the experimental runs developed. Therefore, this work could guide researchers in the efficient use of UDs in the simulation and optimization of virus production processes. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 10/52521-6 - Producao de glicoproteinas da raiva atraves do cultivo de celulas bhk-21 em biorreatores usando sistema de expressao semliki forest virus (sfv).
• Beneficiário: Eutimio Gustavo Fernández Núñez
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado