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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Penicillium echinulatum secretome analysis reveals the fungi potential for degradation of lignocellulosic biomass

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Autor(es):
Hahn Schneider, Willian Daniel [1] ; Goncalves, Thiago Augusto [2, 3] ; Uchima, Cristiane Akemi [2] ; Couger, Matthew Brian [4] ; Prade, Rolf [4] ; Squina, Fabio Marcio [2] ; Pinheiro Dillon, Aldo Jose [1] ; Camassola, Marli [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Caxias do Sul, Inst Biotechnol, Enzymes & Biomass Lab, Francisco Getulio Vargas St 1130, BR-95070560 Caxias Do Sul, RS - Brazil
[2] CNPEM, Lab Nacl Ciencia & Tecnol Bioetanol CTBE, Giuseppe Maximo Scolfaro 10-000, BR-13083970 Campinas, SP - Brazil
[3] State Univ Campinas UNICAMP, Inst Biol, Dept Biochem, Sao Paulo - Brazil
[4] Oklahoma State Univ, Dept Microbiol & Mol Genet, 1110 South Innovat Way, Stillwater, OK 74078 - USA
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: BIOTECHNOLOGY FOR BIOFUELS; v. 9, MAR 17 2016.
Citações Web of Science: 24
Resumo

Background: The enzymatic degradation of lignocellulosic materials by fungal enzyme systems has been extensively studied due to its effectiveness in the liberation of fermentable sugars for bioethanol production. Recently, variants of the fungus Penicillium echinulatum have been described as a great producer of cellulases and considered a promising strain for the bioethanol industry. Results: Penicillium echinulatum, wild-type 2HH and its mutant strain S1M29, were grown on four different carbon sources: cellulose, sugar cane bagasse pretreated by steam explosion (SCB), glucose, and glycerol for 120 h. Samples collected at 24, 96, and 120 h were used for enzymatic measurement, and the 96-h one was also used for secretome analysis by 1D-PAGE LC-MS/MS. A total of 165 proteins were identified, and more than one-third of these proteins belong to CAZy families. Glycosyl hydrolases (GH) are the most abundant group, being represented in larger quantities by GH3, 5, 17, 43, and 72. Cellobiohydrolases, endoglucanases, beta-glycosidases, xylanases, beta-xylosidases, and mannanases were found, and in minor quantities, pectinases, ligninases, and amylases were also found. Swollenin and esterases were also identified. Conclusions: Our study revealed differences in the two strains of P. echinulatum in several aspects in which the mutation improved the production of enzymes related to lignocellulosic biomass deconstruction. Considering the spectral counting analysis, the mutant strain S1M29 was more efficient in the production of enzymes involved in cellulose and hemicellulose degradation, despite having a nearly identical CAZy enzymatic repertoire. Moreover, S1M29 secretes more quantities of protein on SCB than on cellulose, relevant information when considering the production of cellulases using raw materials at low cost. Glucose, and especially glycerol, were used mainly for the production of amylases and ligninases. (AU)

Processo FAPESP: 12/19040-0 - Caracterização da expressão gênica do fungo Neurospora crassa na desconstrução do bagaço de cana de açúcar
Beneficiário:Cristiane Akemi Uchima
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 08/58037-9 - Geração de biblioteca para conversão enzimática de biomassa a partir de metagenoma do solo
Beneficiário:Fábio Márcio Squina
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Apoio a Jovens Pesquisadores
Processo FAPESP: 14/06923-6 - Recalcitrância da biomassa de cana-de-açúcar: fundamentos relacionados à formação da parede celular, ao pré-tratamento e à digestão enzimática, aplicados no desenvolvimento de novos modelos de biorrefinarias
Beneficiário:Andre Luis Ferraz
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Temático