Evaluation of photodynamic therapy on fibroblast viability and cytokine production

Autor(es):Mostrar menos -

Gomes-Filho, Joao Eduardo ^[1] ; Sivieri-Araujo, Gustavo ^[1] ; Sipert, Carla Renata ^[2] ; da Silva Santos, Ludmilla Mota ^[1] ; de Azevedo Queiroz, India Olinta ^[1] ; Martins, Christine Men ^[1] ; do Carmo Maia, Nayara Kivilla ^[1] ; Angelo Cintra, Luciano Tavares ^[1] ; Dezan-Junior, Eloi ^[1] ; Bagnato, Vanderlei Salvador ^[3] ; Chaves-Neto, Antonio Hernandes ^[4] ; Penha de Oliveira, Sandra Helena ^[5]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Aracatuba Sch Dent, Discipline Endodont, Dept Restorat Dent, Aracatuba, SP - Brazil

^[2] Univ Sao Paulo, Sch Dent, Discipline Endodont, Dept Restorat Dent, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Phys Inst Sao Carlos, Opt Grp, Sao Carlos, SP - Brazil

^[4] Sao Paulo State Univ, Aracatuba Sch Dent, Discipline Biochem, Dept Basic Sci, Aracatuba, SP - Brazil

^[5] Sao Paulo State Univ, Aracatuba Sch Dent, Discipline Pharmacol, Dept Basic Sci, Aracatuba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Photodiagnosis and Photodynamic Therapy; v. 13, p. 97-100, MAR 2016.
• Citações Web of Science: 5

Resumo

Background: The aim of this study was to evaluate the effects of photodynamic therapy with curcumin (PDT) comparatively to 5% sodium hypochlorite (NaOCl) and saline solution on cell viability and cytokine (IL-1 beta and IL-6) production by mouse fibroblasts. Methods: Sixty seconds of pre-irradiation time with curcumin 500 mg/L and Led wavelength (lambda) 480 nm, 72 J cm(2), for 300 s was used for PDT. Solutions were diluted in culture medium DMEM (1 x 10(4) cells) and placed into 24-well cell culture plates with mouse fibroblasts L-929. Culture medium was used as control. After 6,24 and 48 h, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay (MIT) was used to evaluate the cell viability and the supernatant was collected for cytokine evaluation using enzyme-linked immunosorbent assay (ELISA). The results were statistically analyzed by ANOVA and BonFerroni correction (p < 0.05) for MIT and Kruskal-Wallis test and Dunn (p < 0.05) for ELISA. Results: PDT and saline solution presented low cytotoxic effect similar to the control group (p > 0.05) while 5% NaOCl was more cytotoxic than PDT (p < 0.05) in all periods of time. All materials similarly expressed IL-1 beta and IL-6 regardless to the experimental period (p < 0.05). Conclusions: PDT with curcumin was not cytotoxic to L929 fibroblasts differently from 5% NaOCl. In all groups occurred similar expression of IL-1 beta and IL-6. (C) 2016 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 12/06785-7 - Influência de diferentes soluções irrigadoras associadas à terapia fotodinâmica: estudo in vitro e in vivo
• Beneficiário: João Eduardo Gomes Filho
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/23287-8 - Influência de diferentes soluções irrigadoras associadas à terapia fotodinâmica: estudo in vitro e in vivo
• Beneficiário: Gustavo Sivieri de Araújo
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 12/12695-0 - Influência de diferentes soluções irrigadoras associadas à terapia fotodinâmica: estudo in vitro da citotoxicidade em fibroblastos L-929
• Beneficiário: Nayara Kívilla Do Carmo Maia
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica