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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of photodynamic therapy on fibroblast viability and cytokine production

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Author(s):
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Gomes-Filho, Joao Eduardo [1] ; Sivieri-Araujo, Gustavo [1] ; Sipert, Carla Renata [2] ; da Silva Santos, Ludmilla Mota [1] ; de Azevedo Queiroz, India Olinta [1] ; Martins, Christine Men [1] ; do Carmo Maia, Nayara Kivilla [1] ; Angelo Cintra, Luciano Tavares [1] ; Dezan-Junior, Eloi [1] ; Bagnato, Vanderlei Salvador [3] ; Chaves-Neto, Antonio Hernandes [4] ; Penha de Oliveira, Sandra Helena [5]
Total Authors: 12
Affiliation:
[1] Sao Paulo State Univ, Aracatuba Sch Dent, Discipline Endodont, Dept Restorat Dent, Aracatuba, SP - Brazil
[2] Univ Sao Paulo, Sch Dent, Discipline Endodont, Dept Restorat Dent, Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Phys Inst Sao Carlos, Opt Grp, Sao Carlos, SP - Brazil
[4] Sao Paulo State Univ, Aracatuba Sch Dent, Discipline Biochem, Dept Basic Sci, Aracatuba, SP - Brazil
[5] Sao Paulo State Univ, Aracatuba Sch Dent, Discipline Pharmacol, Dept Basic Sci, Aracatuba, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Photodiagnosis and Photodynamic Therapy; v. 13, p. 97-100, MAR 2016.
Web of Science Citations: 5
Abstract

Background: The aim of this study was to evaluate the effects of photodynamic therapy with curcumin (PDT) comparatively to 5% sodium hypochlorite (NaOCl) and saline solution on cell viability and cytokine (IL-1 beta and IL-6) production by mouse fibroblasts. Methods: Sixty seconds of pre-irradiation time with curcumin 500 mg/L and Led wavelength (lambda) 480 nm, 72 J cm(2), for 300 s was used for PDT. Solutions were diluted in culture medium DMEM (1 x 10(4) cells) and placed into 24-well cell culture plates with mouse fibroblasts L-929. Culture medium was used as control. After 6,24 and 48 h, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay (MIT) was used to evaluate the cell viability and the supernatant was collected for cytokine evaluation using enzyme-linked immunosorbent assay (ELISA). The results were statistically analyzed by ANOVA and BonFerroni correction (p < 0.05) for MIT and Kruskal-Wallis test and Dunn (p < 0.05) for ELISA. Results: PDT and saline solution presented low cytotoxic effect similar to the control group (p > 0.05) while 5% NaOCl was more cytotoxic than PDT (p < 0.05) in all periods of time. All materials similarly expressed IL-1 beta and IL-6 regardless to the experimental period (p < 0.05). Conclusions: PDT with curcumin was not cytotoxic to L929 fibroblasts differently from 5% NaOCl. In all groups occurred similar expression of IL-1 beta and IL-6. (C) 2016 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 12/06785-7 - Influence of diferente irrigating solutions associated to photodinamic therapy: in vitro and in vivo study
Grantee:João Eduardo Gomes Filho
Support Opportunities: Regular Research Grants
FAPESP's process: 11/23287-8 - Influence of different irrigating solution associated to the photodynamic therapy: in vitro and in vivo study
Grantee:Gustavo Sivieri de Araújo
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 12/12695-0 - Influence of different irrigating solutions associated to photodynamic therapy: in vitro cytotoxicity study in fibroblasts L-929
Grantee:Nayara Kívilla Do Carmo Maia
Support Opportunities: Scholarships in Brazil - Scientific Initiation