Effects of ovariectomy on periodontal tissues following tooth replantation

Autor(es)

Marao, Heloisa Fonseca ^[1] ; Mao, Jeremy J. ^[2] ; Casatti, Claudio Aparecido ^{[3, 4]} ; Coelho, Paulo G. ^{[5, 6, 7]} ; Ervolino, Edilson ^[3] ; Zhou, Jian ^[2] ; da Silva, Vanessa Ferreira ^[1] ; Panzarini, Sonia Regina ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Aracatuba Dent Sch, Dept Surg & Integrated Clin, Rua Jose Bonifacio, 1193 Vila Mendonca, BR-16015050 Aracatuba, SP - Brazil

^[2] Columbia Univ, Coll Dent Med, Ctr Craniofacial Regenerat, 630 West 168th St, New York, NY 10032 - USA

^[3] Univ Estadual Paulista UNESP, Aracatuba Dent Sch, Dept Basic Sci, Rodovia Marechal Rondon, KM 527-528, Campus Univ, BR-16018805 Aracatuba, SP - Brazil

^[4] UNESP Univ Estadual Paulista, Biosci Inst Botucatu, Botucatu, SP - Brazil

^[5] New York Univ Abu Dhabi, Div Engn, Abu Dhabi - U Arab Emirates

^[6] New York Univ, Coll Dent, Dept Biomat & Biomimet, 433 1st Ave, New York, NY 10010 - USA

^[7] NYU, Coll Dent, Dept Periodontol & Implant Dent, 345 E 24th St, New York, NY 10010 - USA

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF ORAL BIOLOGY; v. 65, p. 9-16, MAY 2016.
• Citações Web of Science: 0

Resumo

Objectives: The aim of the study was to analyze the effects of ovariectomy on periodontal tissues following immediate tooth replantation by histomorphometric, immunohistochemistry, and mu CT analysis. Materials and methods: Eighty wistar rats (Rattus norvegicus albinos) with normal estrous cycles were randomly divided into two groups: ovariectomized (OVX) and Sham. Two months after surgery, the rats' upper right incisor was extracted followed by immediate reimplantation. The animals were sacrificed after 28, 45, and 60 days healing time. Histomorphometric and immunohistochemical analysis were performed by evaluation of PCNA and TRAP straining. Results: The periodontal ligament was reinserted into the bone and cementum in the both groups. The immunohistochemical analysis revealed PCNA positive cells on the periodontal ligament in both groups at 28 days. Root resorption was noted at 45 days with immunoreactive cells for TRAP present in bone and tooth surface however no statistical differences between the groups were noticed. Histomorphometric analysis showed significant difference between groups in the periodontal ligament and root resorption parameters for the sub-items: intensity of chronic inflammatory infiltrate at 60 days (p < 0.01), the organization of the periodontal ligament at 28 days (p < 0.05), depth of root resorption at 45 days (p < 0.05) and at 60 days (p < 0.001). The mu CT analysis showed multiple areas of bone resorption in association with OVX at 28 and 60 days with no significant differences between times in vivo. Conclusion: The ovariectomy did not have significant influence in periodontal tissue parameters following tooth reimplantation. (C) 2016 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 10/12593-8 - Efeito da ovariectomia no reimplante dentário: análise histomorfométrica e imunoistoquímica em ratas
• Beneficiário: Heloisa Fonseca Marão
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/09295-8 - Efeito da deficiência de estrógeno no reimplante dentário: análise histomorfométrica e imunoistoquímica em ratas
• Beneficiário: Sonia Regina Panzarini
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/08254-9 - Efeito da ovariectomia no reimplante dentário: análise por micro tomografia computadorizada
• Beneficiário: Heloisa Fonseca Marão
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado