Analysis of DRB3 gene polymorphisms in Jafarabadi, Mediterranean, and Murrah buffaloes from Brazil

Autor(es)

Stafuzza, N. B. ^{[1, 2]} ; Olivatto, L. M. ^[2] ; Naressi, B. C. M. ^[2] ; Tonhati, H. ^[3] ; Amaral-Trusty, M. E. J. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Dept Ciencias Exatas, Fac Ciencias Agr & Vet, Jaboticabal, SP - Brazil

^[2] Univ Estadual Paulista, Dept Biol, Inst Biociencias Letras & Ciencias Exatas, Sao Jose Do Rio Preto - Brazil

^[3] Univ Estadual Paulista, Dept Zootecnia, Fac Ciencias Agr & Vet, Jaboticabal, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Genetics and Molecular Research; v. 15, n. 1 2016.
• Citações Web of Science: 0

Resumo

The DRB3 gene is an MHC class II gene that has a high degree of polymorphism with more than 100 alleles described in cattle. This variation contributes to differences among individuals in immune responsiveness and disease resistance. In this study, we searched for allelic variants in exon 2 of the DRB3 gene in 80 river buffaloes of three breeds in Brazil using a PCR-RFLP technique. The PCR product showed genetic polymorphism when digested with RsaI, PstI or HaeIII restriction enzymes. In total, 16 restriction patterns were identified: nine restriction patterns and 16 genotypes were found with RsaI; four restriction patterns and nine genotypes were found with HaeIII; and, three restriction patterns and four genotypes were found with PstI. Three RFLP patterns were exclusive to Jafarabadi buffaloes (RsaI-b, RsaI-c and RsaI-f) and three others were only observed in Mediterranean buffaloes (RsaI-g, RsaI-h and PstI-y). Jafarabadi buffaloes had a larger number of RFLP patterns than Mediterranean and Murrah breeds. The analysis showed that the DRB3 exon 2 was highly polymorphic, with the highest degree of polymorphism in Mediterranean buffaloes. This study provides the first assessment of allelic variation among three different buffalo breeds from Brazil and provides a basis for further investigations into the association between the DRB3 alleles and disease resistance. (AU)

• Processo FAPESP: 11/11889-3 - Região MHC do búfalo - organização molecular utilizando ferramentas genômicas de última geração
• Beneficiário: Maria Elisabete Jorge Amaral
• Modalidade de apoio: Auxílio à Pesquisa - Regular