Time course of the meiotic arrest in sheep cumulus-oocyte complexes treated with roscovitine

Autor(es)

Crocomo, Leticia Ferrari ^[1] ; Marques Filho, Wolff Camargo ^[1] ; Ackermann, Camila Louise ^[1] ; Paschoal, Daniela Martins ^[1] ; Guastali, Midyan Daroz ^[1] ; Dias Maziero, Rosiara Rosaria ^[1] ; Sudano, Mateus Jose ^[2] ; Landim-Alvarenga, Fernanda da Cruz ^[1] ; Bicudo, Sony Dimas ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Dept Anim Reprod & Vet Radiol, Sch Vet Med & Anim Sci FMVZ, Botucatu, SP - Brazil

^[2] Fed Univ Pampa, Lab Genet & Anim Breeding, Uruguaiana, RS - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ZYGOTE; v. 24, n. 2, p. 310-318, APR 2016.
• Citações Web of Science: 2

Resumo

Temporary meiosis arrest with cyclin-dependent kinases inhibitors has been proposed in order to improve the quality of in vitro matured oocytes. In sheep, however, this phenomenon has been rarely investigated. Therefore, the present study aimed to evaluate the effect of different incubation times with roscovitine on nuclear maturation and cumulus cell expansion of sheep cumulus-oocyte complexes (COCs). For this, COCs were cultured for 0, 6, 12 or 20 h in basic maturation medium (Control) containing 75 M roscovitine (Rosco). After, they were in vitro matured (IVM) for 18 h in the presence of luteinizing hormone (LH) and follicle-stimulating hormone (FSH). At the end of each treatment, cumulus cell expansion and nuclear maturation were assessed under a stereomicroscope and by Hoechst 33342 staining, respectively. In the Control and Rosco groups, the absence of cumulus cell expansion prevailed at 0, 6, 12 and 20 h. After IVM for 18 h, total cumulus cell expansion in the Rosco treatments was dependent on the exposure time to roscovitine. A significantly high percentage of oocytes treated with roscovitine for 6 h (87%), 12 h or 20 h (65%) were arrested at the germinal vesicle (GV) stage. In contrast, 23% GVBD, 54% metaphase I (MI) and 61% MII oocytes were observed in the Control groups at 6, 12 and 20 h, respectively. In all treatments, a significant percentage of oocytes reached MII after IVM for 18 h. Therefore, roscovitine reversibly arrested the meiosis of sheep oocytes during different culture times with the maximal efficiency of meiotic inhibition reached at 6 h. In addition, reversibility of its inhibitory action on cumulus cells was exposure-time dependent. (AU)

• Processo FAPESP: 11/18843-9 - Expressão gênica e potencial de desenvolvimento in vitro dos complexos cumulus-oócitos ovinos tratados com roscovitina
• Beneficiário: Sony Dimas Bicudo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/14041-5 - Expressão gênica e potencial de desenvolvimento in vitro dos complexos cumulus-oócitos ovinos tratados com roscovitina
• Beneficiário: Letícia Ferrari Crocomo
• Modalidade de apoio: Bolsas no Brasil - Doutorado