Cytocompatibility of HEMA-free resin-based luting cements according to application protocols on dentine surfaces

Autor(es)

Soares, D. G. ^[1] ; Brito, C. A. ^[2] ; Tavares da Silva, R. H. B. ^[3] ; Ribeiro, A. P. D. ^[4] ; Hebling, J. ^[5] ; de Souza Costa, C. A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Dept Physiol & Pathol, Araraquara Sch Dent, Humaita St 1680, BR-14801903 Araraquara, SP - Brazil

^[2] Univ Paulista, Dept Dent, Goiania, Go - Brazil

^[3] Univ Estadual Paulista, Araraquara Sch Dent, Dept Dent Mat & Prosthodont, BR-1480190 Araraquara, SP - Brazil

^[4] Fed Univ Brasilia, Dept Dent, Campus Univ Darcy Ribeiro, Brasilia, DF - Brazil

^[5] Univ Estadual Paulista, Araraquara Sch Dent, Dept Orthodont & Pediat Dent, BR-1480190 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Endodontic Journal; v. 49, n. 6, p. 551-560, JUN 2016.
• Citações Web of Science: 6

Resumo

Aim To evaluate the transdentinal cytotoxicity of resin-based luting cements (RBLCs), with no HEMA in their composition, to odontoblast-like cells. Methodology Human dentine discs 0.3 mm thick were adapted to artificial pulp chambers (APCs) and placed in wells of 24-well plates containing 1 mL of culture medium (DMEM). Two categories of HEMA-free RBLCs were evaluated: group 1, self-adhesive Rely X Unicem (RU; 3M ESPE), applied directly to the dentine substrate; and group 2, Rely X ARC (RARC; 3M ESPE), applied to dentine previously acid-etched and treated with a bonding agent. In group 3 (control), considered as representing 100% cell metabolic activity, no treatment was performed on dentine. The APC/disc sets were incubated for 24 h or 7 days at 37 degrees C and 5% CO2. Then, the extracts (DMEM + dental materials components that diffused through dentine) were applied to cultured odontoblast-like MDPC-23 cells for 24 h. After that, the cell viability (MTT assay), cell morphology (SEM), total protein production (TP) and alkaline phosphatase (ALP) activity were assessed. Data from MTT assay and TP production were analysed by Kruskal-Wallis and Mann-Whitney tests (alpha = 5%). Data from ALP activity were analysed by one-way ANOVA and Tukey's test (alpha = 5%). Results In group 1, a slight reduction in cell viability (11.6% and 16.8% for 24-h and 7-day periods, respectively) and ALP activity (13.5% and 17.9% for 24-h and 7-day periods, respectively) was observed, with no significant difference from group 3 (control) (P > 0.05). In group 2, a significant reduction in cell viability, TP production and ALP activity compared with group 3 (control) occurred (P < 0.05), regardless of incubation time. Alteration in MDPC-23 cell morphology was observed only in group 2. Conclusions HEMA-free Rely X ARC cement caused greater toxicity to odontoblast-like MDPC-23 cells than did Rely X Unicem cement when both resin-based luting materials were applied to dentine as recommended by the manufacturer. (AU)

• Processo FAPESP: 13/23520-0 - Bioatividade de scaffolds experimentais a base de quitosana e colágeno sobre cultura de células pulpares humanas
• Beneficiário: Diana Gabriela Soares dos Passos
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/05879-0 - Efeito da laserterapia de baixa potência e fatores de crescimento sobre a atividade de fibroblastos de gengiva obtidos de pacientes jovens e idosos
• Beneficiário: Carlos Alberto de Souza Costa
• Modalidade de apoio: Auxílio à Pesquisa - Regular