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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Tissue-specific distribution of hemicelluloses in six different sugarcane hybrids as related to cell wall recalcitrance

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Autor(es):
Costa, Thales H. F. [1] ; Vega-Sanchez, Miguel E. [2, 3] ; Milagres, Adriane M. F. [1] ; Scheller, Henrik V. [2] ; Ferraz, Andre [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Escola Engn Lorena, Dept Biotecnol, BR-12602810 Lorena, SP - Brazil
[2] Lawrence Berkeley Natl Lab, Joint BioEnergy Inst, 1 Cyclotron Rd, Berkeley, CA 94720 - USA
[3] Monsanto Co, 800N Lindbergh Blvd, St Louis, MO 63141 - USA
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: BIOTECHNOLOGY FOR BIOFUELS; v. 9, MAY 4 2016.
Citações Web of Science: 15
Resumo

Background: Grasses are lignocellulosic materials useful to supply the billion-tons annual requirement for renewable resources that aim to produce transportation fuels and a variety of chemicals. However, the polysaccharides contained in grass cell walls are built in a recalcitrant composite. Deconstruction of these cell walls is still a challenge for the energy-efficient and economically viable transformation of lignocellulosic materials. The varied tissue-specific distribution of cell wall components adds complexity to the origins of cell wall recalcitrance in grasses. This complexity usually led to empirically developed pretreatment processes to overcome recalcitrance. A further complication is that efficient pretreatment procedures generally treat the less recalcitrant tissues more than necessary, which results in the generation of undesirable biomass degradation products. Results: Six different sugarcane hybrids were used as model grasses to evaluate the tissue-specific distribution of hemicelluloses and the role of these components in cell wall recalcitrance. Acetylated glucuronoarabinoxylan (GAX) occurs in all tissues. Mixed-linkage glucan (MLG) was relevant in the innermost regions of the sugarcane internodes (up to 15.4 % w/w), especially in the low-lignin content hybrids. Immunofluorescence microscopy showed that xylans predominated in vascular bundles, whereas MLG occurred mostly in the parenchyma cell walls from the pith region of the hybrids with low-lignin content. Evaluation of the digestibility of sugarcane polysaccharides by commercial enzymes indicated that the cell wall recalcitrance varied considerably along the internode regions and in the sugarcane hybrids. Pith regions of the hybrids with high MLG and low-lignin contents reached up to 85 % cellulose conversion after 72 h of hydrolysis, without any pretreatment. Conclusions: The collective characteristics of the internode regions were related to the varied recalcitrance found in the samples. Components such as lignin and GAX were critical for the increased recalcitrance, but low cellulose crystallinity index, high MLG contents, and highly substituted GAX contributed to the generation of a less recalcitrant material. (AU)

Processo FAPESP: 11/50535-2 - Análise micro-espectrofotométrica aplicada ao estudo da recalcitrância em materiais lignocelulósicos
Beneficiário:Andre Luis Ferraz
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 08/56256-5 - Topochemistry, porosity and chemical composition determining successful enzymatic sacarification of sugarcane bagasse
Beneficiário:Adriane Maria Ferreira Milagres
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Temático
Processo FAPESP: 14/06923-6 - Recalcitrância da biomassa de cana-de-açúcar: fundamentos relacionados à formação da parede celular, ao pré-tratamento e à digestão enzimática, aplicados no desenvolvimento de novos modelos de biorrefinarias
Beneficiário:Andre Luis Ferraz
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Temático